Browsing by Author "Kartal, Melis"

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Citation - WoS: 18
Apoptotic Effects of Resveratrol, a Grape Polyphenol, on Imatinib-Sensitive and Resistant K562 Chronic Myeloid Leukemia Cells
(International Institute of Anticancer Research, 2012-07) Can, Geylani; Baran, Yusuf; Çakır, Zeynep; Kartal, Melis; Gündüz, Ufuk; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
To examine the antiproliferative and apoptotic effects of resveratrol on imatinib-sensitive and imatinib-resistant K562 chronic myeloid leukemia cells. Antiproliferative effects of resveratrol were determined by the 3-Bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT) cell proliferation assay. Apoptotic effects of resveratrol on sensitive K562 and resistant K562/IMA-3 cells were determined through changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP), and apoptosis by annexin V-(FITC). The concentrations of resveratrol that inhibited cell growth by 50% (IC(50)) were calculated as 85 and 122 μM for K562 and K562/IMA-3 cells, respectively. There were 1.91-, 7.42- and 14.73-fold increases in loss of MMP in K562 cells treated with 10, 50, and 100 μM resveratrol, respectively. The same concentrations of resveratrol resulted in 2.21-, 3.30- and 7.65-fold increases in loss of MMP in K562/IMA-3 cells. Caspase-3 activity increased 1.04-, 2.77- and 4.8-fold in K562 and 1.02-, 1.41- and 3.46-fold in K562/IMA-3 cells in response to the same concentrations of resveratrol, respectively. Apoptosis was induced in 58.7%- and 43.3% of K562 and K562/IMA-3 cells, respectively, in response to 100 μM resveratrol. Taken together these results may suggest potential use of resveratrol in CML, as well as in patients with primary and/or acquired resistance to imatinib.
Ceramides Regulate Resveratrol-Induced Apoptosis in Human Acute Promyelocytic and Chronic Myeloid Leukemia Cells
(Ferrata Storti Foundation, 2010) Baran, Yusuf; Baran, Yusuf; Kartal, Melis; Çakır, Zeynep; Saydam, Güray; Şahin, Fahri; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
[No abstract available]
A Natural Product, Quercetin Enhances the Cytotoxic and Apoptotic Effects of Dexamethasone on Jurkat Cells
(Ferrata Storti Foundation, 2012) Baran, Yusuf; Baran, Yusuf; Kartal, Melis; Kozanoğlu, İlknur; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
[No abstract available]
Citation - WoS: 30
Citation - Scopus: 35
Quercetin-Induced Apoptosis Involves Increased Htert Enzyme Activity of Leukemic Cells
(Taylor and Francis Ltd., 2011-09) Avcı, Çığır Biray; Ekiz, Hüseyin Atakan; Yılmaz, Sunde; Baran, Yusuf; Doğan, Zeynep Özlem; Saydam, Güray; Dodurga, Yavuz; Ekiz, Hüseyin Atakan; Kartal, Melis; Şahin, Fahri; Baran, Yusuf; Gündüz, Cumhur; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
We aimed to examine the growth suppressive effects of quercetin on acute promyelocytic and lymphoblastic leukemia and chronic myeloid leukemia, and to find out whether the growth suppression is related to the blocking of telomerase enzyme activity. Cytotoxic effects of quercetin were shown by trypan blue analyses. Apoptotic effects of quercetin were examined by acridine orange and ethidium bromide staining by fluorescence microscopy. The effects of quercetin on telomerase enzyme activity were shown by hTERT Quantification Kit. Our results demonstrated that quercetin has antiproliferative and apoptotic effects on T-cell acute lymphoblastic leukemia (ALL), acute promyelocytic leukemia, and chronic myeloid leukemia (CML) cells. We also showed for the first time by this study that quercetin suppresses the activity of telomerase in ALL and CML cells. The results of this study show the importance of quercetin for its therapeutic potential in treatment of leukemias.
Therapeutic Potential of Targeting Sphingolipid Signaling Pathways in Various Types of Cancers
(Elsevier Ltd., 2010) Baran, Yusuf; Baran, Yusuf; Başsoy, Esen Yonca; Ekiz, Hüseyin Atakan; Çakır, Zeynep; Camgöz, Aylin; Gencer, Emel Başak; Kartal, Melis; Can, Geylani; Ekiz, Hüseyin Atakan; Güçlüler, Gözde; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
[No abstract available]