Browsing by Author "Kaya, Zehra"

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Citation - WoS: 3
Citation - Scopus: 5
Assessing the Impact of Non-Thermal and Thermal Treatment on the Shelf-Life of Onion Juice
(Czech Academy of Agricultural Sciences, 2018) Demir, Hande; Yıldız, Mustafa Kemal; Becerikli, İsmail; Ünlütürk, Sevcan; Kaya, Zehra; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Onion (Allium cepa L.) juice is a marinating agent for meat and fish marination and readily usable sauce for any meal that has onion in its formulation. This study aims to assess the microbiological and physicochemical changes in the onion juice processed by UV-C irradiation (0.5 mm sample depth, 30 min exposure time, 7.5 mW/cm(2) UV incident intensity) and conventional heat treatment (74.5 degrees C, 12 min) during its storage. Microbiological results showed processing by UV-C irradiation or heat treatment under optimum conditions extended the microbial shelf-life of untreated onion juice by minimum 6-times. Total colour change of heat-treated samples was lower than that of untreated and UV-C treated samples for 12 weeks. Also, pH, total titratable acidity, total soluble solids content, turbidity, NEBI and total phenolic content were monitored for 12 weeks. The results of this study will form scientific infrastructure for onion juice manufacturers to decide on the processing method with respect to its shelf-life.
Development of Different Koruk (unripe Grape) Products by Using Several Processing Techniques
(Izmir Institute of Technology, 2018-07) Kaya, Zehra; Ünlütürk, Sevcan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Verjuice, obtained by pressing unripe grapes, is mostly used as an alternative to lemon juice and vinegar in salads and traditional meals due to its unique sour taste and flavour. It has a short shelf life due to growth of yeasts and molds when produced at household conditions. Verjuice products in the market are not meeting desirable quality characteristics. The high quality verjuice and its derivatives required to be produced by considering several processing techniques. The objectives of this Ph.D. thesis are to (i) produce long shelf life verjuice with minimum change in its “fresh like” properties by using the combination of UV-C irradiation or Pulsed-UV light (PUV) with mild heating, (ii) produce verjuice powder with a good storage stability by using freeze drying, (iii) produce concentrated verjuice with minimal quality loss by using vacuum evaporation. Verjuice was successfully pasteurized without loosing its desirable quality by a combined UV-C and mild heating (78.0 mJ/mL, 6.2 min, 51.2°C) and PUV assisted with mild heating (6.12 J/cm2, 8.5 min, 47°C) and mild thermal (72°C, 18 s) treatments. 5-log reduction of target S. cerevisiae (NRRL Y-139) was aimed for the pasteurization of verjuice. No microorganism was developed in pasteurized verjuice during 12 and 6 weeks of refrigerated storage after UV+MH and PUV+MH treatments, respectively. Freezedried verjuice powder containing maltodextrin (FD 48h, 20% MD), was highly stable under the accelerated storage conditions (40°C, 90%RH) for 70 days. Concentrated verjuice obtained under vacuum conditions of 45°C/913 mbar, showed minimal quality change and was consumable up to 12 weeks at refrigerated storage.
Disinfection of White Grape Juice by Using Continuous Flow Uv Reactor
(Izmir Institute of Technology, 2011) Kaya, Zehra; Ünlütürk, Sevcan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Although continuous flow UV irradiation process is applied to several kind of fruit juices as a non-thermal method, it hasnot treated to white grape juice before. The main objective was to study the effect of commercial UV disinfection system on the spoilage microorganisms in white grape juice. Physical, chemical and optical properties were measured after and before irradiation. Biodosimetric studies were performed by using Bench Top UV apparatus and continuous flow commercial UV reactor. Bench top UV study showed that 228.96 mJ/cm2 UV dose provided complete inactivation of S. cerevisiae in the pasteurized juice at 0.91 mW/cm2 UV intensity.. 3.00±0.16 log cfu/ml yeasts and 4.32±0.03 log cfu/ml lactic acid bacteria reduction were detected in naturally fermented juice after 282.24 mJ/cm2 UV dose. D(10) of yeasts (43.86 mJ/cm2) were higher than lactic acid bacteria (33.78 mJ/cm2) because of showing higher resistance to UV light. In the continuous flow UV reactor, the highest reduction of S. cerevisiae was achieved as 3.39±0.044 log cfu/ml at 56.06 mJ/cm2 UV dose and low flow rate (820 ml/min) in pasteurized juice. However, highest reduction was observed in yeast and lactic acid bacteria count as 1.70±0.028 log cfu/ml and 2.49±0.0031 log cfu/ml respectively at 19.96 mJ/cm2 UV dose in medium flow rate (1516 ml/min) of fresh squeezed juice. Absorbance, color, and titratable acidity were changed by irradiation. As a result of 13 days shelf life study, no significant increase was seen in spoilage microorganisms after irradiation.
Citation - WoS: 79
Citation - Scopus: 100
Effect of Uv-C Irradiation and Heat Treatment on the Shelf Life Stability of a Lemon-Melon Juice Blend: Multivariate Statistical Approach
(Elsevier Ltd., 2015-05) Kaya, Zehra; Yıldız, Semanur; Ünlütürk, Sevcan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Heat treatment and UV-C irradiation of lemon and melon juice (LMJ) blends were comparatively evaluated by examining their impact on E. coli K12 (ATCC 25253) and their physicochemical properties, i.e., total soluble solids (TSS), pH, titratable acidity (TA), color, turbidity and absorbance coefficient, both immediately after processing and during 30 days of refrigerated storage. The newly formulated LMJ blend containing 12% (v/v) lemon juice (pH 3.92 ± 0.01) scored the highest in the consumer acceptance test. Upon UV-C irradiation (2.461 J/mL) and heat treatment (72 °C, 71 s), the E. coli K12 population in LMJ blend was reduced by > 6 log10 CFU/mL. Principal component analysis (PCA) and hierarchical cluster analyses (HCA) showed a clear discrimination among the physicochemical properties of the control and the UV-C and heat-treated LMJ blends during storage, suggesting that UV-C irradiation has a comparable effect on microbial stability at 4 °C and better quality preservation performance than heat treatment. Industrial relevance Melon juice has many beneficial health effects. It has high sugar content, pH (5.6-6.0) and a fairly short shelf life. Therefore, pasteurization is required. But the thermal pasteurization has some undesired effects on the juice quality. Consumer demands for high quality fruit juice with fresh-like characteristics has markedly expanded in recent years. In this study, an alternative lemon-melon juice (LMJ) blend formulation was developed, and pasteurized using both UV-C irradiation and mild heat treatment. The shelf life stability of pasteurized LMJ blends was assessed by means of principal component analysis and hierarchical cluster analysis. The shelf life of LMJ blends treated by both methods was increased from 2 days to 30 days. The multivariate data analysis was successfully applied as a tool for an overall evaluation of the shelf-life of the product. UV-C irradiation has a comparable effect on microbial stability at 4 °C and better quality preservation performance than heat treatment for obtaining both shelf-stable and fresh-like LMJ blends. This would be a major advantage in processing of nutritious juice products.
Citation - WoS: 20
Citation - Scopus: 26
Effectiveness of Pulsed Light Treatments Assisted by Mild Heat on Saccharomyces Cerevisiae Inactivation in Verjuice and Evaluation of Its Quality During Storage
(Elsevier, 2020) Martin Belloso, Olga; Soliva Fortuny, Robert; Kaya, Zehra; Ünlütürk, Sevcan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
The effects of pulsed light (PL) processing parameters such as depth of juice layer (1, 3, 5 mm), distance from the lamp (5, 10 cm) and number of pulses (0-50 pulses) on the inactivation of Saccharomyces cerevisiae in verjuice, a clarified beverage obtained from freshly-squeezed unripe grapes, were investigated. A reduction of 0.96 +/- 0.27 log CFU/mL was achieved by applying a dose of 34 J/cm(2) (1-mm layer depth, 5-cm distance, 50 pulses). PL was combined with mild heating (MH) at 43, 45 and 47 degrees C to increase its inactivation efficacy. Pasteurization was achieved by applying 17 J/cm(2) at 45 degrees C (PLMH45-3) and 6.12 J/cm(2) at 47 degrees C (PLMH47-3) to a 3-mm juice layer with S. cerevisiae reductions of 5.10 +/- 0.24 and 5.06 +/- 0.08 log CFU/mL, respectively. Quality properties of PLMH47-3-pasteurized verjuice were monitored during 6 weeks of storage at refrigerated (5 degrees C) and room temperature (25 degrees C), The results were compared to those of untreated and thermally pasteurized (72 degrees C/18 s) samples. Untreated juice spoiled within 2 weeks at 25 degrees C. No growth was detected in other conditions for 6 weeks. Among quality characteristics, only optical properties changed slightly during storage. It was concluded that mild MH-assisted pulsed light treatments have potential for verjuice pasteurization compared to conventional thermal pasteurization due to the better preservation of its fresh-like characteristics.
Impact of Pasteurization Process on the Quality and Marination Properties of Onion Juice
(2020) Kaya, Zehra; Yıldız, Mustafa Kemal; Demir, Hande; Unluturk, Sevcan; Becerikli, İsmail; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
This study aims to compare UV-C irradiation and conventional heat treatment to produce pasteurizedonion juice used as a meat marinating agent. The process conditions maximizing the inactivation oftarget microorganism Escherichia coli K-12 were; 0.5 mm sample depth, 30 min irradiation, 7.5mW/cm2 UV incident intensity for UV-C and, 74.5°C and 12 min for heat treatment. Except pH andnon-enzymatic browning index, differences between physicochemical properties of raw, UV-C andheat-treated onion juices were significant. Springiness and chewiness of unmarinated beefsteakswere higher compared to the ones marinated with the fresh and pasteurized onion juice (UV-C andheat). Pasteurization of onion juice (UV-C and heat) did not significantly affect general liking scorescompared to beefsteaks marinated in untreated onion juice.
Citation - WoS: 16
Citation - Scopus: 22
Pasteurization of Verjuice by Uv-C Irradiation and Mild Heat Treatment
(Wiley, 2019) Kaya, Zehra; Ünlütürk, Sevcan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Verjuice is a highly acidic juice and more prone to yeast spoilage. In this study, the efficacy of individual and combination processes of UV-C irradiation (UV) and mild heat treatment (MH) for pasteurization of verjuice were assessed. Saccharomyces cerevisiae (NRRL Y-139) was selected as the target microorganism and kinetic parameters for MH, UV, and combined UV + MH inactivation treatments were determined. The UV treatment alone at a UV dose of 0.57 J/cm(2) (energy of 2.30 J/mL), provided only 0.54 +/- 0.02 log CFU/mL reduction of S. cerevisiae. In contrast, the combined treatment (UV + MH2) substantially reduced the number of S. cerevisiae in verjuice, 5.16 +/- 0.24 log CFU/mL reduction was achieved at 0.25 J/cm(2) UV dose (energy of 1.01 J/mL) and 51.25 +/- 1.47 degrees C. The percentage of synergism for the UV + MH inactivation of S. cerevisiae in verjuice was maximized at 51.25 degrees C (50.79% of synergistic effect). Inactivation kinetics of S. cerevisiae was best described by Weibull model with the smallest RMSE and AIC values. D value was decreased from 13.66 to 1.94 min when UV was combined with mild heating. The results showed that UV-C light assisted by mild heat treatment can be a potential alternative to thermal pasteurization of verjuice. Practical applications Fruit juices are prone to spoilage by yeasts, molds, and some acid-tolerant bacteria. Saccharomyces cerevisiae is a heat resistant spoilage microorganism and found in some spoiled juices. Thermal pasteurization is widely used for the preservation of fruit juices but results in losses of essential nutrients and changes in physicochemical and organoleptic properties. This study illustrated that the combined UV-C light assisted by mild heat treatment can deliver the required microbial reduction in verjuice. The synergistic effect of two processing methods is suggested for controlling the growth of spoilage microflora of fruit juices.
Citation - Scopus: 8
Uv Processing and Storage of Liquid and Solid Foods: Quality, Microbial, Enzymatic, Nutritional, Organoleptic, Composition and Properties Effects
(Elsevier, 2021) Hakgüder Taze, Bengi; Pelvan Akgün, Merve; Yıldız, Semanur; Kaya, Zehra; Ünlütürk, Sevcan; 01. Izmir Institute of Technology; 03.08. Department of Food Engineering; 03. Faculty of Engineering
Non-thermal food processing technologies have been explored extensively in recent years in order to develop food products with extended shelf life as well as preserved nutritional and organoleptic characteristics in accordance with the changing consumer demands (Falguera et al., 2011a; Sanchez-Moreno et al., 2009). Ultraviolet (UV) irradiation is one of the non-thermal processes that can be applied to reduce the microbial load in liquid foods and surfaces, and to sterilize food packages and packaging materials, and environments involved in food processes (Jimenez-Sanchez et al., 2017a; Bintsis et al., 2000). UV light is subdivided into three regions as short-wave UV (UV-C, 200 and 280 nm), medium-wave UV (UV-B, 280 to 315 nm), and long wave UV (UV-A, 315 to 400 nm). The different types of effects on microorganisms can be caused by UV light of different wavelengths. The effectiveness of UV light on microorganisms results primarily from the fact that DNA molecules absorb UV photons between 200 and 300 nm, with peak absorption around 260–265 nm. This causes DNA damage by altering the nucleotide base pairing, thereby creating new linkages between adjacent nucleotides, particularly between pyrimidine bases, on the same DNA strand and ultimately results in cell death (Zimmer and Slawson, 2002). Peak et al. (1984) proposed that the dimer formation is not the only requirement to damage the DNA. Absorption of different wavelength photons by different molecular groups in the long DNA molecule can damage or destroy these bond groups. Thus, different bonds in the DNA can be affected with photons of different energy (Neister, 2014).