Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10243
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dc.contributor.authorÖnal, Sevgi-
dc.contributor.authorTürker Burhan, Merve-
dc.contributor.authorBatı Ayaz, Gizem-
dc.contributor.authorYanık, Hamdullah-
dc.contributor.authorPesen Okvur, Devrim-
dc.date.accessioned2021-01-24T18:33:13Z-
dc.date.available2021-01-24T18:33:13Z-
dc.date.issued2021-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://doi.org/10.1016/j.biomaterials.2020.120412-
dc.identifier.urihttps://hdl.handle.net/10243-
dc.description.abstractBreast cancer cells (BCC) and macrophages are known to interact via epidermal growth factor (EGF) produced by macrophages and colony stimulating factor-1 (CSF-1) produced by BCC. Despite contradictory findings, this interaction is perceived as a paracrine loop. Further, the underlying mechanism of interaction remains unclear. Here, we investigated interactions of BCC with macrophages in 2D and 3D. While both BCC and macrophages showed invasion/chemotaxis to fetal bovine serum, only macrophages showed chemotaxis to BCC in custom designed 3D cell-on-a-chip devices. These results were in agreement with gradient simulation results and ELISA results showing that macrophage-derived-EGF was not secreted into macrophage-conditioned-medium. Live cell imaging of BCC in the presence and absence of iressa showed that macrophages but not macrophage-derived-matrix modulated adhesion and motility of BCC in 2D. 3D co-culture experiments in collagen and matrigel showed that BCC changed their multicellular organization in the presence of macrophages. In custom designed 3D co-culture cell-on-a-chip devices, macrophages promoted and reduced migration of BCC in collagen and matrigel, respectively. Furthermore, adherent but not suspended BCC endocytosed EGFR when in contact with macrophages. Collectively, our data revealed that macrophages showed chemotaxis towards BCC whereas BCC required direct contact to interact with macrophage-derived-EGF. Therefore, we propose that the interaction between cancer cells and macrophages is a paracrine-juxtacrine loop of CSF-1 and EGF, respectively. © 2020 Elsevier Ltden_US
dc.description.sponsorshipThis work was supported by FP7 Marie Curie Grant number PIRG08-GA-2010-276976 and IYTE Scientific Research Project Grant number 2011IYTE25 (to Devrim Pesen-Okvur). Cell-on-a-chips were fabricated at the IYTE Applied Quantum Research Center, supported by DPT ( State Planning Organization ) Grant 2009K120860 .en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiomaterialsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast cancer cellsen_US
dc.subjectCell-on-a-chipen_US
dc.subjectEpidermal growth factoren_US
dc.subjectJuxtacrineen_US
dc.subjectMacrophagesen_US
dc.subjectParacrineen_US
dc.titleBreast cancer cells and macrophages in a paracrine-juxtacrine loopen_US
dc.typeArticleen_US
dc.institutionauthorÖnal, Sevgi-
dc.institutionauthorTürker Burhan, Merve-
dc.institutionauthorBatı Ayaz, Gizem-
dc.institutionauthorYanık, Hamdullah-
dc.institutionauthorPesen Okvur, Devrim-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume267en_US
dc.identifier.wosWOS:000598391300005en_US
dc.identifier.scopus2-s2.0-85095772627en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.biomaterials.2020.120412-
dc.identifier.pmid33161320en_US
dc.relation.doi10.1016/j.biomaterials.2020.120412en_US
dc.coverage.doi10.1016/j.biomaterials.2020.120412en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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