Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10263
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dc.contributor.authorNartop, Pınar-
dc.contributor.authorGürel, Aynur-
dc.contributor.authorAkgün, İsmail Hakkı-
dc.contributor.authorBedir, Erdal-
dc.date.accessioned2021-01-24T18:33:23Z-
dc.date.available2021-01-24T18:33:23Z-
dc.date.issued2019-
dc.identifier.issn1303-5002-
dc.identifier.issn2687-475X-
dc.identifier.urihttps://hdl.handle.net/10263-
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/375541-
dc.description.abstractI n this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate the effects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV (1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1 and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however, astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol (5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectivelyen_US
dc.description.abstractBu çalışmada, Astragalus trojanus Stev. gövde ve yaprak eksplantlarından elde edilen iki gram ağırlığında kalluslar 1 mg/L 2,4-D içeren Woody Plant Medium (WPM) besin ortamında dört hafta boyunca kültüre alınmışlar ve çalışma hacminin ve besin ortamının etkilerinin incelenmesi için başlangıç materyali (inokulum) olarak kullanılmışlardır. En fazla biyokütle astragaloside IV (1.66 µg/mg) ve cycloastragenol (0.19 g/mg) içeriğiyle 250 ml’lik erlenden elde edilmiştir. Farklı konsantrasyonlarda sükroz ve kazein hidrolizat (1 ve 2 g/L) denemeleri yapılmış ve pH’ın etkisi de incelenmiştir. Biyokütle birikiminde artış sağlanamamış ancak astragaloside IV ve cycloastragenol içerikleri artmıştır. En yüksek astragaloside IV (95.23 µg/mg) ve cycloastragenol (5.93 µg/mg) içerikleri pH 6.8’de ve 2 g/L kazein hidrolizat denemesinde elde edilmiştir.en_US
dc.language.isoenen_US
dc.publisherHacettepe Üniversitesien_US
dc.relation.ispartofHacettepe Journal of Biology and Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAstragalus trojanus-
dc.subjectBatch culture-
dc.subjectCycloastragenol.-
dc.titleAstragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysateen_US
dc.title.alternativeAstragalus trojanus Stev. Kesikli Kültürleri: pH, Sükroz ve Kazein Hidrolizat Etkisi ile Sikloartan-tip Metabolit Birikimien_US
dc.typeArticleen_US
dc.institutionauthorBedir, Erdal-
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume47en_US
dc.identifier.issue2en_US
dc.identifier.startpage185en_US
dc.identifier.endpage192en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.trdizinid375541en_US
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
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