Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10328
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dc.contributor.authorSeyrantepe, Volkan-
dc.contributor.authorDelman, Murat-
dc.date.accessioned2021-01-24T18:33:54Z-
dc.date.available2021-01-24T18:33:54Z-
dc.date.issued2014-
dc.identifier.issn1300-0152-
dc.identifier.issn1303-6092-
dc.identifier.urihttps://hdl.handle.net/10328-
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/163072-
dc.identifier.urihttps://doi.org/10.3906/biy-1401-63-
dc.description.abstractThere are 4 different sialidases that have been described in humans: lysosomal (Neu1), cytoplasmic (Neu2), plasma membrane (Neu3), and lysosomal/mitochondrial (Neu4). Previously, we have shown that Neu4 has a broad substrate specificity and is active against glyco-conjugates, including GM2 ganglioside, at the acidic pH of 3.2. An overexpression of Neu4 in transfected neuroglia cells from a Tay-Sachs patient shows a clearance of accumulated GM2, indicating the biological importance of Neu4. In this paper, we aimed to characterize a minimal promoter region of the human Neu4 gene in order to understand the molecular mechanism regulating its expression. We cloned 7 different DNA fragments from the human Neu4 promoter region into luciferase expression vectors for a reporter assay and also performed an electrophoretic mobility shift assay to demonstrate the binding of transcription factors. We demonstrated that -187 bp upstream of the Neu4 gene is a minimal promoter region for controlling transcription from the human Neu4 gene. The electrophoretic mobility shift assay showed that the minimal promoter region recruits a c-myc transcription factor, which might be responsible for regulation of Neu4 gene transcription. The data we obtained might be useful to discover small molecules, which control selective high expression of the human Neu4 gene, resulting in the normal morphological phenotype in the lysosomes of Tay-Sachs patients.en_US
dc.language.isoenen_US
dc.publisherTÜBİTAK - Türkiye Bilimsel ve Teknolojik Araştırma Kurumuen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSialidasesen_US
dc.subjectProtein-DNA interactionen_US
dc.subjectTranscription factorsen_US
dc.titleCharacterization of the human sialidase Neu4 gene promoteren_US
dc.typeArticleen_US
dc.institutionauthorSeyrantepe, Volkan-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume38en_US
dc.identifier.issue5en_US
dc.identifier.startpage574en_US
dc.identifier.endpage580en_US
dc.identifier.wosWOS:000341523900003en_US
dc.identifier.scopus2-s2.0-84906860458-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3906/biy-1401-63-
dc.identifier.trdizinid163072en_US
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ2-
item.languageiso639-1en-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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