Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10380
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dc.contributor.authorGökçe, Begümtr
dc.contributor.authorAkçok, İsmailtr
dc.contributor.authorÇağır, Alitr
dc.contributor.authorPesen Okvur, Devrimtr
dc.date.accessioned2021-01-24T18:34:22Z-
dc.date.available2021-01-24T18:34:22Z-
dc.date.issued2020-
dc.identifier.issn0928-0987-
dc.identifier.issn1879-0720-
dc.identifier.urihttps://doi.org/10.1016/j.ejps.2020.105542-
dc.identifier.urihttps://hdl.handle.net/10380-
dc.descriptionPubMed: 32927074en_US
dc.description.abstractDrug discovery has a 90% rate of failure because preclinical platforms for drug testing do not mimic the in vivo conditions. Doxorubicin (DOX) is a commonly used drug to treat breast cancer patients even though it has side effects. Lab-on-a-chip (LOC) devices provide spatial control at the micrometer scale and can thus emulate the cancer microenvironment. Here, using a multidisciplinary approach, a new drug testing platform based on 3D tri-culture in LOC devices was developed. Breast cancer cells alone or with normal mammary epithelial cells and macrophages were cultured in matrigel in LOC devices. The platform was used to test DOX and (R)-4'-methylklavuzon (KLA), which is a new anti-cancer drug candidate. Results showed that DOX and KLA were equally effective on breast cancer cells in 3D monoculture. KLA produced 26% less death for breast cancer cells than DOX in 3D tri-culture. More importantly, DOX was not selective between breast cancer cells and normal mammary epithelial cells in 3D tri- culture whereas KLA caused 56% less cell death than DOX for normal mammary epithelial cells. Results strongly recommend that 3D tri-culture in LOC devices be used for assessment of drug toxicity at the preclinical stage.en_US
dc.description.sponsorshipThis work was supported by Turkish Academy of Sciences -The Young Scientists Award 2016, The Science Academy Society of Turkey Young Scientists Award 2016 and Izmir Institute of Technology - 2017G_IYTE97 research grants awarded to Devrim Pesen Okvur. Samples were fabricated in the IYTE Applied Quantum Research Center, supported by State Planning Organization -Grant 2009K120860. Synthesis was supported by the Scientific and Technological Research Council of Turkey -TUBITAK 110T782 grant to Ali Cagir. We thank Katherine Willcox Ozsari from Izmir Institute of Technology Academic Writing Center for assisting in English language editing.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciencesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDrug discoveryen_US
dc.subjectBreast cancer cellen_US
dc.subject3D cell cultureen_US
dc.subjectTri-culture, doxorubicinen_US
dc.subjectLab-on-a-chipen_US
dc.titleA new drug testing platform based on 3D tri-culture in lab-on-a-chip devicesen_US
dc.typeArticleen_US
dc.institutionauthorGökçe, Begümtr
dc.institutionauthorAkçok, İsmailtr
dc.institutionauthorÇağır, Alitr
dc.institutionauthorPesen Okvur, Devrimtr
dc.departmentİzmir Institute of Technology. Chemistryen_US
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume155en_US
dc.identifier.wosWOS:000582710600016en_US
dc.identifier.scopus2-s2.0-85091361547en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.ejps.2020.105542-
dc.identifier.pmid32927074en_US
dc.relation.doi10.1016/j.ejps.2020.105542en_US
dc.coverage.doi10.1016/j.ejps.2020.105542en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.01. Department of Chemistry-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Chemistry / Kimya
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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