Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10422
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dc.contributor.authorKıvrak, Ezgitr
dc.contributor.authorİnce Yardımcı, Atiketr
dc.contributor.authorİlhan, Receptr
dc.contributor.authorBallar Kırmızıbayrak, Petektr
dc.contributor.authorYılmaz, Selahattintr
dc.contributor.authorKara, Pınartr
dc.date.accessioned2021-01-24T18:43:08Z-
dc.date.available2021-01-24T18:43:08Z-
dc.date.issued2020-
dc.identifier.issn1618-2642-
dc.identifier.issn1618-2650-
dc.identifier.urihttps://doi.org/10.1007/s00216-020-02916-x-
dc.identifier.urihttps://hdl.handle.net/10422-
dc.description.abstractIn the present study, a sensitive electrochemical aptamer-based biosensing strategy for human non-small cell lung cancer (NSCLC) detection was proposed using nanofiber-modified disposable pencil graphite electrodes (PGEs). The composite nanofiber was comprised of polyacrylonitrile (PAN) and polypyrrole (PPy) polymers, and fabrication of the nanofibers was accomplished using electrospinning process onto PGEs. Development of the nanofibers was confirmed using scanning electron microscopy (SEM). The high-affinity 5 '-aminohexyl-linked aptamer was immobilized onto a PAN/PPy composite nanofiber-modified sensor surface via covalent bonding strategy. After incubation with NSCLC living cells (A549 cell line) at 37.5 degrees C, the recognition between aptamer and target cells was monitored by electrochemical impedance spectroscopy (EIS). The selectivity of the aptasensor was evaluated using nonspecific human cervical cancer cells (HeLa) and a nonspecific aptamer sequence. The proposed electrochemical aptasensor showed high sensitivity toward A549 cells with a detection limit of 1.2 x 10(3)cells/mL. The results indicate that our label-free electrochemical aptasensor has great potential in the design of aptasensors for the diagnostics of other types of cancer cells with broad detection capability in clinical analysis.en_US
dc.description.sponsorshipThe authors acknowledge financial support from Ege University, Graduate School of Natural and Applied Science, Project Coordinator (18/FBE/001). We also acknowledge the technical support from the Pharmaceutical Sciences Research Center (FABAL), Faculty of Pharmacy, Ege University.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptasensoren_US
dc.subjectEarly cancer diagnosisen_US
dc.subjectNon-small cell lung canceren_US
dc.subjectElectrochemical impedance spectrometryen_US
dc.subjectNanofibersen_US
dc.titleAptamer-based electrochemical biosensing strategy toward human non-small cell lung cancer using polyacrylonitrile/polypyrrole nanofibersen_US
dc.typeArticleen_US
dc.institutionauthorİnce Yardımcı, Atiketr
dc.institutionauthorYılmaz, Selahattintr
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume412en_US
dc.identifier.issue28en_US
dc.identifier.startpage7851en_US
dc.identifier.endpage7860en_US
dc.identifier.wosWOS:000569549000002en_US
dc.identifier.scopus2-s2.0-85091002284en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr
dc.identifier.doi10.1007/s00216-020-02916-x-
dc.identifier.pmid32935151en_US
dc.relation.doi10.1007/s00216-020-02916-xen_US
dc.coverage.doi10.1007/s00216-020-02916-xen_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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