Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10473
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKaradaş, Özge-
dc.contributor.authorMeşe, Gülistan-
dc.contributor.authorÖzçivici, Engin-
dc.date.accessioned2021-01-24T18:44:52Z-
dc.date.available2021-01-24T18:44:52Z-
dc.date.issued2020-
dc.identifier.issn2093-9868-
dc.identifier.issn2093-985X-
dc.identifier.urihttps://doi.org/10.1007/s13534-020-00161-w-
dc.identifier.urihttps://hdl.handle.net/10473-
dc.descriptionPubMed: 32850178en_US
dc.description.abstractAnabolic effects of low magnitude high frequency (LMHF) vibrations on bone tissue were consistently shown in the literature in vivo, however in vitro efforts to elucidate underlying mechanisms are generally limited to 2D cell culture studies. Three dimensional cell culture platforms better mimic the natural microenvironment and biological processes usually differ in 3D compared to 2D culture. In this study, we used laboratory grade filter paper as a scaffold material for studying the effects of LHMF vibrations on osteogenesis of bone marrow mesenchymal stem cells in a 3D system. LMHF vibrations were applied 15 min/day at 0.1 g acceleration and 90 Hz frequency for 21 days to residing cells under quiescent and osteogenic conditions. mRNA expression analysis was performed for alkaline phosphatase (ALP) and osteocalcin (OCN) genes, Alizarin red S staining was performed for mineral nodule formation and infrared spectroscopy was performed for determination of extracellular matrix composition. The highest osteocalcin expression, mineral nodule formation and the phosphate bands arising from the inorganic phase was observed for the cells incubated in osteogenic induction medium with vibration. Our results showed that filter paper can be used as a model scaffold system for studying the effects of mechanical loads on cells, and LMHF vibrations induced the osteogenic differentiation of stem cells.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215S862]; Turkish Academy of SciencesTurkish Academy of Sciencesen_US
dc.description.sponsorshipFinancial support from The Scientific and Technological Research Council of Turkey (215S862-EO) and Turkish Academy of Sciences (Young Investigator Award-EO) is gratefully acknowledged. We are thankful for the helpful discussions with Ozden Yalcn-Ozuysal, PhD. The Izmir Institute of Technology, Biotechnology and Bioengineering Research Center is also appreciated for instrumental support.en_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofBiomedical Engineering Lettersen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVibrationen_US
dc.subjectFilter paperen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectScaffolden_US
dc.subjectOsteogenic differentiationen_US
dc.titleLow magnitude high frequency vibrations expedite the osteogenesis of bone marrow stem cells on paper based 3D scaffoldsen_US
dc.typeArticleen_US
dc.institutionauthorKaradaş, Özge-
dc.institutionauthorMeşe, Gülistan-
dc.institutionauthorÖzçivici, Engin-
dc.departmentIzmir Institute of Technology. Biotechnology and Bioengineerinen_US
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.identifier.startpage431en_US
dc.identifier.endpage441en_US
dc.identifier.wosWOS:000545879800001en_US
dc.identifier.scopus2-s2.0-85087644376en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s13534-020-00161-w-
dc.identifier.pmid32850178en_US
dc.relation.doi10.1007/s13534-020-00161-wen_US
dc.coverage.doi10.1007/s13534-020-00161-wen_US
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.cerifentitytypePublications-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

1
checked on Dec 11, 2021

WEB OF SCIENCETM
Citations

2
checked on Jan 15, 2022

Page view(s)

16
checked on Jan 17, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.