Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10725
Title: Evaluation of Malassezia Species by Fourier Transform Infrared (FT-IR) Spectroscopy
Authors: Ergin, Çağrı
Vuran, M. Emre
Gök, Yaşar
Özdemir, Durmuş
Karaarslan, Aydın
Kaleli, İlnur
Çon, Ahmet Hilmi
Keywords: Malassezia species
FT-IR spectroscopy
taxonomy
identification
Issue Date: 2011
Publisher: Ankara Microbiology Society
Abstract: Malassezia species which are lipophilic exobasidiomycetes fungi, have been accepted as members of normal cutaneous flora as well as causative agent of certain skin diseases. In routine microbiology laboratory, species identification based on phenotypic characters may not yield identical results with taxonomic studies. Lipophilic and lipid-dependent Malassezia yeasts require lipid-enriched complex media. For this reason, Fourier transform infrared (FT-IR) spectroscopy analysis focused on lipid window may be useful for identification of Malassezia species. In this study, 10 different standard Malassezia species (M.dermatis CBS 9145, M.furfur CBS 7019, M.japonica CBS 9432, M.globosa CBS 7966, M.nana CBS 9561, M.obtusa CBS 7876, M.pachydermatis CBS 1879, M.slooffiae CBS 7956, M.sympodialis CBS 7222 and M.yamatoensis CBS 9725) which are human pathogens, have been analyzed by FT-IR spectroscopy following standard cultivation onto modified Dixon agar medium. Results showed that two main groups (M1; M.globosa, Robtusa, M.sympodialis, M.dermatis, M.pachydermatis vs, M2; M.furfur, M.japonica, M.nana, M.slooffiae, M.yamatoensis) were discriminated by whole spectra analysis. M.obtusa in M1 by 1686-1606 cm(-1) wavenumber ranges and M.japonicum in M2 by 2993-2812 cm(-1) wavenumber ranges were identified with low level discrimination power. Discriminatory areas for species differentiation of M1 members as M.sympodialis, M.globosa and M.pachydermatis and M2 members as M.furfur and M.yamatoensis could not be identified. Several spectral windows analysis results revealed that FT-IR spectroscopy was not sufficient for species identification of culture grown Malassezia species.
Description: PubMed: 22090301
URI: https://hdl.handle.net/11147/10725
ISSN: 0374-9096
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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