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https://hdl.handle.net/11147/11372
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Anıl İnevi, Müge | - |
dc.contributor.author | Delikoyun, Kerem | - |
dc.contributor.author | Meşe Özçivici, Gülistan | - |
dc.contributor.author | Tekin, Hüseyin Cumhur | - |
dc.contributor.author | Özçivici, Engin | - |
dc.date.accessioned | 2021-11-06T09:47:00Z | - |
dc.date.available | 2021-11-06T09:47:00Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.issn | 1097-0290 | - |
dc.identifier.uri | https://doi.org/10.1002/bit.27941 | - |
dc.identifier.uri | https://hdl.handle.net/11147/11372 | - |
dc.description.abstract | Diamagnetic levitation is an emerging technology for remote manipulation of cells in cell and tissue level applications. Low-cost magnetic levitation configurations using permanent magnets are commonly composed of a culture chamber physically sandwiched between two block magnets that limit working volume and applicability. This work describes a single ring magnet-based magnetic levitation system to eliminate physical limitations for biofabrication. Developed configuration utilizes sample culture volume for construct size manipulation and long-term maintenance. Furthermore, our configuration enables convenient transfer of liquid or solid phases during the levitation. Before biofabrication, we first calibrated/ the platform for levitation with polymeric beads, considering the single cell density range of viable cells. By taking advantage of magnetic focusing and cellular self-assembly, millimeter-sized 3D structures were formed and maintained in the system allowing easy and on-site intervention in cell culture with an open operational space. We demonstrated that the levitation protocol could be adapted for levitation of various cell types (i.e., stem cell, adipocyte and cancer cell) representing cells of different densities by modifying the paramagnetic ion concentration that could be also reduced by manipulating the density of the medium. This technique allowed the manipulation and merging of separately formed 3D biological units, as well as the hybrid biofabrication with biopolymers. In conclusion, we believe that this platform will serve as an important tool in broad fields such as bottom-up tissue engineering, drug discovery and developmental biology. | en_US |
dc.description.sponsorship | Turkiye Bilimsel ve Teknolojik Arastirma Kurumu, Grant/Award Number: 119M755 | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.ispartof | Biotechnology And Bioengineering | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Biofabrication | en_US |
dc.subject | Cellular spheroids | en_US |
dc.subject | Magnetic levitation | en_US |
dc.subject | Scaffold free | en_US |
dc.subject | Stem cells | en_US |
dc.title | Magnetic Levitation Assisted Biofabrication, Culture, and Manipulation of 3d Cellular Structures Using a Ring Magnet Based Setup | en_US |
dc.type | Article | en_US |
dc.authorid | 0000-0003-2854-3472 | - |
dc.authorid | 0000-0003-4464-0475 | - |
dc.authorid | 0000-0002-3294-049X | - |
dc.department | İzmir Institute of Technology. Bioengineering | en_US |
dc.department | İzmir Institute of Technology. Molecular Biology and Genetics | en_US |
dc.identifier.wos | WOS:000703410900001 | en_US |
dc.identifier.scopus | 2-s2.0-85116344892 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1002/bit.27941 | - |
dc.identifier.pmid | 34559409 | en_US |
dc.authorwosid | Anil Inevi, Muge/K-1025-2016 | - |
dc.identifier.wosquality | Q2 | - |
dc.identifier.scopusquality | Q2 | - |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
crisitem.author.dept | 04.03. Department of Molecular Biology and Genetics | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
Appears in Collections: | Bioengineering / Biyomühendislik Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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File | Size | Format | |
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Biotech Bioengineering-2021.pdf | 8.71 MB | Adobe PDF | View/Open |
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