Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11426
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dc.contributor.authorÖz, Yasin-
dc.contributor.authorSürmeli, Yusuf-
dc.contributor.authorŞanlı Mohamed, Gülşah-
dc.date.accessioned2021-11-06T09:48:31Z-
dc.date.available2021-11-06T09:48:31Z-
dc.date.issued2022-08-
dc.identifier.issn0885-4513-
dc.identifier.issn1470-8744-
dc.identifier.urihttps://doi.org/10.1002/bab.2213-
dc.identifier.urihttps://hdl.handle.net/11147/11426-
dc.description.abstractThe immobilization of the biocatalysts onto magnetic nanoparticles has been extensively applied as the external magnetic field facilitates the enzyme recovery from the reaction mixture. In the present study, glutaraldehyde-modified magnetite-cornstarch nanoparticles (MCNs) were successfully synthesized, elaborately characterized by ZetaSizer and surface-enhanced Raman spectroscopy, and used for the immobilization of a thermoalkalophilic esterase from Geobacillus sp. The optimal immobilization conditions were obtained at 65 degrees C, 2:3 molar ratios of Fe2+:Fe3+, and 1 g cornstarch resulted in approximately 90 nm magnetic particles in size. Also, immobilization yield and immobilization efficiency of the esterase were found as 74% and 82%, respectively. Scanning electron microscopy micrographs showed that MCNs were uniform, spherical in shape, and well dispersed and esterase immobilized MCNs displayed similar morphology as free MCNs. The maximum activity of free and immobilized esterase was obtained at 65 degrees C and pH 9. Immobilization onto glutaraldehyde-modified MCNs significantly enhanced the esterase thermostability. Additionally, the immobilized esterase kept its residual activity of 75% after three sequential cycles, suggesting that it has favorable operational stability.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology and Applied Biochemistryen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGeobacillus spen_US
dc.subjectImmobilizationen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectThermoalkalophilic esteraseen_US
dc.titleEnhanced thermostability of the immobilized thermoalkalophilic esterase onto magnetic-cornstarch nanoparticleen_US
dc.typeArticleen_US
dc.departmentİzmir Institute of Technology. Chemistryen_US
dc.identifier.wosWOS:000667267800001en_US
dc.identifier.scopus2-s2.0-85136513398en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/bab.2213-
dc.identifier.pmid34151468en_US
dc.authorwosidSURMELI, YUSUF/AAR-3671-2020-
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ3-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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