Please use this identifier to cite or link to this item:
https://hdl.handle.net/11147/11436
Title: | PCR-Free methodology for detection of single-nucleotide polymorphism with a cationic polythiophene reporter | Authors: | Yücel, Müge Koç, Altuğ UÜgenalp, Ayfer Akkoç, Gün Deniz Ceyhan, Metin Yıldız, Ümit Hakan |
Keywords: | Conjugated polyelectrolyte PCR-free SNP detection Familial Mediterranean fever DNA biosensors Nucleic acid assay |
Publisher: | American Chemical Society | Abstract: | This study presents a nonamplification-based nucleic acid assay for the detection of single-nucleotide polymorphism (SNP) associated with familial Mediterranean fever (FMF) besides polymerase chain reaction (PCR)-based methodologies. The major objective is to show the potential of the proposed assay for rapid screening of FMF in a Mediterranean region of 400 million population. The assay relies on binding difference of specially designed wild and mutant primers to the target genomic DNA, followed by determination of unbound primers by quick titration of a cationic polythiophene reporter. The fluorescent reporter exhibits signal transition from 525 to 580 nm in the presence of unbound primers, and it correlates the binding affinity of label-free primers to the homozygous wild and mutant genomes. As a proof of concept, 26 real samples are studied relying on the ON and OFF fluorescence signals of the cationic polythiophene reporter. The results are analyzed by principal component analysis (PCA), which provides clear separation of healthy and patient individuals. The further analysis by support vector machine (SVM) classification has revealed that our assay converges to 96% overall accuracy. These results support that the PCR-free nucleic acid assay has a significant potential for rapid and cost-effective screening of familial Mediterranean fever. | URI: | https://doi.org/10.1021/acssensors.0c02130 https://hdl.handle.net/11147/11436 |
ISSN: | 2379-3694 |
Appears in Collections: | Chemistry / Kimya PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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acssensors.0c02130.pdf | 2.47 MB | Adobe PDF | View/Open |
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