Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11442
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dc.contributor.authorKara, Aylin-
dc.contributor.authorKoçtürk, Semra-
dc.contributor.authorBilici, Gökçen-
dc.contributor.authorHavıtçıoğlu, Hasan-
dc.date.accessioned2021-11-06T09:49:31Z-
dc.date.available2021-11-06T09:49:31Z-
dc.date.issued2021-
dc.identifier.issn0885-3282-
dc.identifier.issn1530-8022-
dc.identifier.urihttps://doi.org/10.1177/0885328220981189-
dc.identifier.urihttps://hdl.handle.net/11147/11442-
dc.description.abstractTissue engineering approaches which include a combination of cells and scaffold materials provide an alternative treatment for meniscus regeneration. Decellularization and recellularization techniques are potential treatment options for transplantation. Maintenance of the ultrastructure composition of the extracellular matrix and repopulation with cells are important factors in constructing a biological scaffold and eliminating immunological reactions. The aim of the study is to develop a method to obtain biological functional meniscus scaffolds for meniscus regeneration. For this purpose, meniscus tissue was decellularized by our modified method, a combination of physical, chemical, and enzymatic methods and then recellularized with a meniscal cell population composed of fibroblasts, chondrocytes and fibrochondrocytes that obtained from mesenchymal stem cells. Decellularized and recellularized meniscus scaffolds were analysed biochemically, biomechanically and histologically. Our results revealed that cellular components of the meniscus were successfully removed by preserving collagen and GAG structures without any significant loss in biomechanical properties. Recellularization results showed that the meniscal cells were localized in the empty lacuna on the decellularized meniscus, and also well distributed and proliferated consistently during the cell culture period (p < 0.05). Furthermore, a high amount of DNA, collagen, and GAG contents (p < 0.05) were obtained with the meniscal cell population in recellularized meniscus tissue. The study demonstrates that our decellularization and recellularization methods were effective to develop a biological functional meniscus scaffold and can mimic the meniscus tissue with structural and biochemical features. We predict that the obtained biological meniscus scaffolds may provide avoidance of adverse immune reactions and an appropriate microenvironment for allogeneic or xenogeneic recipients in the transplantation process. Therefore, as a promising candidate, the obtained biological meniscus scaffolds might be verified with a transplantation experiment.en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Dokuz Eylul University, Department of Scientific Research Project (2015.KB. SAG.039).en_US
dc.language.isoenen_US
dc.publisherSAGE Publicationsen_US
dc.relation.ispartofJournal of Biomaterials Applicationsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMeniscusen_US
dc.subjectDecellularizationen_US
dc.subjectRecellularizationen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectFibrochondrocytesen_US
dc.subjectTissue engineeringen_US
dc.titleDevelopment of biological meniscus scaffold: Decellularization method and recellularization with meniscal cell population derived from mesenchymal stem cellsen_US
dc.typeArticleen_US
dc.authorid0000-0001-8302-913X-
dc.authorid0000-0001-7528-1845-
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume35en_US
dc.identifier.issue9en_US
dc.identifier.startpage1192en_US
dc.identifier.endpage1207en_US
dc.identifier.wosWOS:000631243600001en_US
dc.identifier.scopus2-s2.0-85099460380en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1177/0885328220981189-
dc.identifier.pmid33444085en_US
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ2-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.dept01.01. Units Affiliated to the Rectorate-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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