Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11570
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dc.contributor.authorÖzsoylu, Dua-
dc.contributor.authorIsık, Tuğba-
dc.contributor.authorDemir, Mustafa M.-
dc.contributor.authorSchoning, Michael J.-
dc.contributor.authorWagner, Torsten-
dc.date.accessioned2021-11-06T09:54:40Z-
dc.date.available2021-11-06T09:54:40Z-
dc.date.issued2021-
dc.identifier.issn0956-5663-
dc.identifier.issn1873-4235-
dc.identifier.urihttps://doi.org/10.1016/j.bios.2021.112983-
dc.identifier.urihttps://hdl.handle.net/11147/11570-
dc.description.abstractAn efficient preservation of a cell-based biosensor chip to achieve a ready-to-use on-site system is still very challenging as the chip contains a living component such as adherent mammalian cells. Herein, we propose a strategy called on-sensor cryopreservation (OSC), which enables the adherent cells to be preserved by freezing (-80 degrees C) on a biosensor surface, such as the light-addressable potentiometric sensor (LAPS). Adherent cells on rigid surfaces are prone to cryo-injury; thus, the surface was modified to enhance the cell recovery for OSC. It relies on i) the integration of elastic electrospun fibers composed of polyethylene vinyl acetate (PEVA), which has a high thermal expansion coefficient and low glass-transition temperature, and ii) the treatment with O-2 plasma. The modified sensor is integrated into a microfluidic chip system not only to decrease the thermal mass, which is critical for fast thawing, but also to provide a precisely controlled micro-environment. This novel cryo-chip system is effective for keeping cells viable during OSC. As a proof-of-concept for the applicability of a ready-to-use format, the extracellular acidification of cancer cells (CHO-K1) was evaluated by differential LAPS measurements after thawing. Results show, for the first time, that the OSC strategy using the cryo-chip allows label-free and quantitative measurements directly after thawing, which eliminates additional post-thaw culturing steps. The freezing of the chips containing cells at the manufacturing stage and sending them via a cold-chain transport could open up a new possibility for a ready-to-use on-site system.en_US
dc.description.sponsorshipDua Ozsoylu (D O) would like to acknowledge the PhD research scholarship grant from Scientific and Technological Research Council of Turkey (TUBITAK) under BIDEB 2214 -A. The authors also gratefully thank the Federal Ministry of Education and Research of Germany (Opto-Switch FKZ: 13N12585). This study is a part of PhD thesis study of DO (thesis number DEU. HSI. PhD- 2013970198) in Dokuz Eylul University, Institute of Health Sciences, Turkey. Furthermore, the authors would like to thank H. Iken for the LAPS chip processing, D. Rolka for the support in SEM and AFM image analysis, B. Schneider for 3D-printed structures and Th. Schnitzler for the support in cell culturing.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOn-sensor cryopreservationen_US
dc.subjectCryo-chipen_US
dc.subjectReady-to-useen_US
dc.subjectLight-addressable potentiometric sensoren_US
dc.subjectChemical imagingen_US
dc.subjectExtracellular acidificationen_US
dc.titleCryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applicationsen_US
dc.typeArticleen_US
dc.authorid0000-0001-7328-9819-
dc.authorid0000-0003-1309-3990-
dc.institutionauthorIsık, Tuğba-
dc.institutionauthorDemir, Mustafa M.-
dc.departmentİzmir Institute of Technology. Materials Science and Engineeringen_US
dc.identifier.volume177en_US
dc.identifier.wosWOS:000618826300006en_US
dc.identifier.scopus2-s2.0-85100030060en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.bios.2021.112983-
dc.identifier.pmid33535119en_US
dc.authorwosidSchoening, Michael/AAN-6364-2021-
dc.authorwosidISIK, TUGBA/A-4012-2017-
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.09. Department of Materials Science and Engineering-
Appears in Collections:Materials Science and Engineering / Malzeme Bilimi ve Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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