Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11581
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dc.contributor.authorKıvrak, Ezgi-
dc.contributor.authorPauzaite, Tekle-
dc.contributor.authorCopeland, Nikki A.-
dc.contributor.authorHardy, John G.-
dc.contributor.authorKara, Pınar-
dc.contributor.authorFırlak, Melike-
dc.contributor.authorİnce Yardımcı, Atike-
dc.contributor.authorYılmaz, Selahattin-
dc.contributor.authorPalaz, Fahreddin-
dc.date.accessioned2021-11-06T09:54:42Z-
dc.date.available2021-11-06T09:54:42Z-
dc.date.issued2021-
dc.identifier.issn2079-6374-
dc.identifier.urihttps://doi.org/10.3390/bios11010017-
dc.identifier.urihttps://hdl.handle.net/11147/11581-
dc.description.abstractThe CRISPR-Cas9 system has facilitated the genetic modification of various model organisms and cell lines. The outcomes of any CRISPR-Cas9 assay should be investigated to ensure/improve the precision of genome engineering. In this study, carbon nanotube-modified disposable pencil graphite electrodes (CNT/PGEs) were used to develop a label-free electrochemical nanogenosensor for the detection of point mutations generated in the genome by using the CRISPR-Cas9 system. Carbodiimide chemistry was used to immobilize the 5 '-aminohexyl-linked inosine-substituted probe on the surface of the sensor. After hybridization between the target sequence and probe at the sensor surface, guanine oxidation signals were monitored using differential pulse voltammetry (DPV). Optimization of the sensitivity of the nanogenoassay resulted in a lower detection limit of 213.7 nM. The nanogenosensor was highly specific for the detection of the precisely edited DNA sequence. This method allows for a rapid and easy investigation of the products of CRISPR-based gene editing and can be further developed to an array system for multiplex detection of different-gene editing outcomes.en_US
dc.description.sponsorshipWe thank the Royal Society for a Newton International Fellowship (NF151479) for Melike Firlak, and a Research Grant (RG160449) for John Hardy. We thank the Lancaster University Faculty of Science and Technology for a Distinguished Visitor Program Grant to support collaboration with Mehmet Ozsoz. We thank North West Cancer Research NWCR for project funding for Tekle Pauzaite (CR1071) and an independent research fellowship for Nikki Copeland (CR879).en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofBiosensorsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCRISPR-Cas9en_US
dc.subjectHomology-directed repair (HDR)en_US
dc.subjectElectrochemical genosensoren_US
dc.subjectMutation detectionen_US
dc.subjectCarbon nanotube-modified PGEen_US
dc.titleDetection of CRISPR-Cas9-Mediated mutations using a carbon nanotube-modified electrochemical genosensoren_US
dc.typeArticleen_US
dc.authoridÖzsöz, Mehmet-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume11en_US
dc.identifier.issue1en_US
dc.identifier.wosWOS:000609857900001en_US
dc.identifier.scopus2-s2.0-85099996617en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3390/bios11010017-
dc.identifier.pmid33429883en_US
dc.identifier.wosqualityN/A-
dc.identifier.scopusqualityQ2-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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