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https://hdl.handle.net/11147/11581
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DC Field | Value | Language |
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dc.contributor.author | Kıvrak, Ezgi | - |
dc.contributor.author | Pauzaite, Tekle | - |
dc.contributor.author | Copeland, Nikki A. | - |
dc.contributor.author | Hardy, John G. | - |
dc.contributor.author | Kara, Pınar | - |
dc.contributor.author | Fırlak, Melike | - |
dc.contributor.author | İnce Yardımcı, Atike | - |
dc.contributor.author | Yılmaz, Selahattin | - |
dc.contributor.author | Palaz, Fahreddin | - |
dc.date.accessioned | 2021-11-06T09:54:42Z | - |
dc.date.available | 2021-11-06T09:54:42Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.uri | https://doi.org/10.3390/bios11010017 | - |
dc.identifier.uri | https://hdl.handle.net/11147/11581 | - |
dc.description.abstract | The CRISPR-Cas9 system has facilitated the genetic modification of various model organisms and cell lines. The outcomes of any CRISPR-Cas9 assay should be investigated to ensure/improve the precision of genome engineering. In this study, carbon nanotube-modified disposable pencil graphite electrodes (CNT/PGEs) were used to develop a label-free electrochemical nanogenosensor for the detection of point mutations generated in the genome by using the CRISPR-Cas9 system. Carbodiimide chemistry was used to immobilize the 5 '-aminohexyl-linked inosine-substituted probe on the surface of the sensor. After hybridization between the target sequence and probe at the sensor surface, guanine oxidation signals were monitored using differential pulse voltammetry (DPV). Optimization of the sensitivity of the nanogenoassay resulted in a lower detection limit of 213.7 nM. The nanogenosensor was highly specific for the detection of the precisely edited DNA sequence. This method allows for a rapid and easy investigation of the products of CRISPR-based gene editing and can be further developed to an array system for multiplex detection of different-gene editing outcomes. | en_US |
dc.description.sponsorship | We thank the Royal Society for a Newton International Fellowship (NF151479) for Melike Firlak, and a Research Grant (RG160449) for John Hardy. We thank the Lancaster University Faculty of Science and Technology for a Distinguished Visitor Program Grant to support collaboration with Mehmet Ozsoz. We thank North West Cancer Research NWCR for project funding for Tekle Pauzaite (CR1071) and an independent research fellowship for Nikki Copeland (CR879). | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.relation.ispartof | Biosensors | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | CRISPR-Cas9 | en_US |
dc.subject | Homology-directed repair (HDR) | en_US |
dc.subject | Electrochemical genosensor | en_US |
dc.subject | Mutation detection | en_US |
dc.subject | Carbon nanotube-modified PGE | en_US |
dc.title | Detection of CRISPR-Cas9-Mediated mutations using a carbon nanotube-modified electrochemical genosensor | en_US |
dc.type | Article | en_US |
dc.authorid | Özsöz, Mehmet | - |
dc.department | İzmir Institute of Technology. Chemical Engineering | en_US |
dc.identifier.volume | 11 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.wos | WOS:000609857900001 | en_US |
dc.identifier.scopus | 2-s2.0-85099996617 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.3390/bios11010017 | - |
dc.identifier.pmid | 33429883 | en_US |
dc.identifier.wosquality | N/A | - |
dc.identifier.scopusquality | Q2 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.dept | 03.02. Department of Chemical Engineering | - |
Appears in Collections: | Chemical Engineering / Kimya Mühendisliği PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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biosensors-11-00017.pdf | 2.16 MB | Adobe PDF | View/Open |
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