Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/11831
Full metadata record
DC FieldValueLanguage
dc.contributor.authorÇetin, Arif E.-
dc.contributor.authorTopkaya, Seda Nur-
dc.contributor.authorYalçın Özuysal, Özden-
dc.contributor.authorKhademhosseini, Ali-
dc.date.accessioned2021-12-02T18:16:17Z-
dc.date.available2021-12-02T18:16:17Z-
dc.date.issued2021-
dc.identifier.issn1936-0851-
dc.identifier.issn1936-086X-
dc.identifier.urihttps://doi.org/10.1021/acsnano.1c04031-
dc.identifier.urihttps://hdl.handle.net/11147/11831-
dc.description.abstractAccessing cell growth on adhesive substrates is critical for identifying biophysical properties of cells and their therapeutic response to drug therapies. However, optical techniques have low sensitivity, and their reliability varies with cell type, whereas microfluidic technologies rely on cell suspension. In this paper, we introduced a plasmonic functional assay platform that can precisely measure cell weight and the dynamic change in real-time for adherent cells. Possessing this ability, our platform can determine growth rates of individual cells within only 10 mm to map the growth profile of populations in short time intervals. The platform could successfully determine heterogeneity within the growth profile of populations and assess subpopulations exhibiting distinct growth profiles. As a proof of principle, we investigated the growth profile of MCF-7 cells and the effect of two intracellular metabolisms critical for their proliferation. We first investigated the negative effect of serum starvation on cell growth. We then studied ornithine decarboxylase (ODC) activity, a key enzyme which is involved in proliferation, and degraded under low osmolarity that inhibits cell growth. We successfully determined the significant distinction between growth profiles of MCF-7 cells and their ODC-overproducing variants that possess strong resistance to the negative effects of low osmolarity. We also demonstrated that an exogenous parameter, putrescine, could rescue cells from ODC inhibition under hypoosmotic conditions. In addition to the ability of accessing intracellular activities through ex vivo measurements, our platform could also determine therapeutic behaviors of cancer cells in response to drug treatments. Here, we investigated difluoromethylornithine (DFMO), which has antitumor effects on MCF-7 cells by inhibiting ODC activity. We successfully demonstrated the susceptibility of MCF-7 cells to such drug treatment, while its DFMO-resistant subpopulation could survive in the presence of this antigrowth agent. By rapidly determining cell growth kinetics in small samples, our plasmonic platform may be of broad use to basic research and clinical applications.en_US
dc.description.sponsorshipA.E.C. acknowledges The Scientific and Technological Research Council of Turkey (TUBITAK) 3501 -Career Development Program (Project No. 119E111) and BAGEP Award of the Science Academy, Turkey. We thank Alper Bagriyanik and Nevin Ersoy for SEM imaging of the plasmonic chips and MCF-7 cells. We also thank Buket Onder for the illustrations.en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofACS Nanoen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPlasmonicsen_US
dc.subjectLabel free biosensingen_US
dc.subjectCell growth profilingen_US
dc.subjectFunctional assaysen_US
dc.subjectCellular metabolismen_US
dc.subjectNanohole arraysen_US
dc.titleRefractive index sensing for measuring single cell growthen_US
dc.typeArticleen_US
dc.authorid0000-0003-0552-368X-
dc.institutionauthorYalçın Özuysal, Özden-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume15en_US
dc.identifier.issue6en_US
dc.identifier.startpage10710en_US
dc.identifier.endpage10721en_US
dc.identifier.wosWOS:000665748900135en_US
dc.identifier.scopus2-s2.0-85108145629en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1021/acsnano.1c04031-
dc.identifier.pmid34029478en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File SizeFormat 
acsnano.1c04031.pdf4.67 MBAdobe PDFView/Open
Show simple item record



CORE Recommender

SCOPUSTM   
Citations

10
checked on Mar 22, 2024

WEB OF SCIENCETM
Citations

11
checked on Mar 27, 2024

Page view(s)

514
checked on Mar 25, 2024

Download(s)

1,558
checked on Mar 25, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.