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Title: Colorimetric and fluorometric profiling of advanced glycation end products
Authors: Ammanath, Gopal
Delachi, Carla Giorgia
Karabacak, Soner
Ali, Yusuf
Boehm, Bernhard O.
Yıldız, Ümit Hakan
Alagappan, Palaniappan
Liedberg, Bo
Nanyang Technological University
Nanyang Technological University
01. Izmir Institute of Technology
Nanyang Technological University
Nanyang Technological University
01. Izmir Institute of Technology
Nanyang Technological University
Nanyang Technological University
Keywords: Advanced glycation end products (AGEs)
Issue Date: Jan-2022
Publisher: American Chemical Society
Abstract: Profiling of advanced glycation end products (AGEs) is an emerging area of clinical significance for disease diagnosis and prognosis. Typically, concentrations of AGEs are estimated in laboratories by trained personnel using sophisticated equipment. Herein, a facile approach for colorimetric and fluorometric profiling of AGEs is reported for rapid and on-site analysis. The concentrations of AGE levels in plasma are estimated via changes in optical properties of polythiophenes (PTs) upon interaction with aptamers (Apts) in the presence and in the absence of AGEs. To validate the proposed approach, glyceraldehyde-derived AGEs (AGE class 1 [AGE1]), the biomarker associated with cardiovascular diseases and diabetes, are used as a model system. Colorimetric analysis yielded linear responses for AGE1 for clinically relevant concentration ranges between 1.5 and 300 μg/mL with a limit of detection (LOD) of ∼1.3 μg/mL. Subsequently, an approach utilizing PTs with four different pendant groups in conjunction with four different Apts is demonstrated for qualitative colorimetric profiling and for quantitative fluorometric profiling of up to four AGEs in clinical matrices. Principal component analysis (PCA) of fluorometric responses of AGE-spiked samples yielded distinct responses for the different AGEs tested. Thus, the proposed approach ascertains rapid profiling of spiked AGEs in plasma samples without the requirement of preanalytical processing and advanced instrumentation, thereby facilitating on-site diagnosis.
Description: The authors wish to acknowledge funding support from Tier 1, MOE -RG 82/12, and NITHM Exploratory Research grant M4081989.070.
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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