Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12284
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dc.contributor.authorGül, Aytenen_US
dc.contributor.authorDöşkaya, Merten_US
dc.contributor.authorCan, Hüseyinen_US
dc.contributor.authorKarakavuk, Muhammeten_US
dc.contributor.authorAnıl İnevi, Mügeen_US
dc.contributor.authorSağlam Metiner, Pelinen_US
dc.contributor.authorAtalay Sahar, Esraen_US
dc.date.accessioned2022-08-09T10:43:41Z-
dc.date.available2022-08-09T10:43:41Z-
dc.date.issued2022-04-
dc.identifier.issn0264-410X-
dc.identifier.urihttps://doi.org/10.1016/j.vaccine.2022.03.014-
dc.identifier.urihttps://hdl.handle.net/11147/12284-
dc.description.abstractBreast cancer was ranked first in global cancer incidence in 2020, and HER2 overexpression in breast cancer accounts for 20–30% of breast cancer patients. Current therapeutic strategies increase the survival rate, but resistance to them occurs frequently, and there is an urgent need to develop novel treatments such as DNA vaccines which can induce a specific and long-lasting immune response against HER2 antigens. To enhance the immunogenicity of DNA vaccines, dendritic cells (DCs) can be targeted using multi-epitope proteins that provide accurate immune focusing. For this purpose, we generated a DNA vaccine encoding a fusion protein composed of 1) in silico discovered antigenic epitopes of human and rat HER2 proteins (MeHer2) and 2) a single-chain antibody fragment (ScFv) specific for the DC-restricted antigen-uptake receptor DEC205 (ScFvDEC). The xenogeneic multi-epitope DNA vaccine (pMeHer2) encodes three only T-cell epitopes, two only B-cell epitopes, and two T and B cell epitopes, and pScFvDEC-MeHer2 vaccine additionally encodes ScFvDEC introduced at the N terminus of the MeHer2. Then, mouse groups were immunized with pScFvDEC-MeHer2, pMeHer2, pScFvDEC, pEmpty, and PBS to determine the elicited immune response. pScFvDEC-MeHer2 vaccinated mice showed a strong IgG response (P < 0.0001) and pScFvDEC-MeHer2 induced a significant IgG2a increase (P < 0.01). The percentages of both IFN-γ secreting CD4 and CD8 T cells were higher in mice immunized with pScFvDEC-MeHer2 compared with the pMeHer2. pScFvDEC-MeHer2 and pMeHer2 secreted significantly higher levels of extracellular IFN-γ compared with to control groups (P < 0.0001). In addition, the IFN-γ level of the pScFvDEC-MeHer2 vaccine group was approximately two times higher than the pMeHer2 group (P < 0.0001). Overall, this study identified the pScFvDECMeHer2 construct as a potential DNA vaccine candidate, supporting further studies to be conducted on HER2+ animal models.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofVaccineen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectBreast canceren_US
dc.subjectDC-targeting vaccineen_US
dc.subjectDNA vaccineen_US
dc.subjectHER2en_US
dc.subjectXenogeneicen_US
dc.titleImmunogenicity of a Xenogeneic Multi-Epitope Her2+ Breast Cancer Dna Vaccine Targeting the Dendritic Cell Restricted Antigen-Uptake Receptor Dec205en_US
dc.typeArticleen_US
dc.authorid0000-0003-2854-3472en_US
dc.institutionauthorAnıl İnevi, Mügeen_US
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.wosWOS:000790939000009en_US
dc.identifier.scopus2-s2.0-85126524139en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.vaccine.2022.03.014-
dc.identifier.pmid35305824-
dc.contributor.affiliationEge Üniversitesien_US
dc.contributor.affiliationEge Üniversitesien_US
dc.contributor.affiliationEge Üniversitesien_US
dc.contributor.affiliationEge Üniversitesien_US
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.contributor.affiliationEge Üniversitesien_US
dc.contributor.affiliationEge Üniversitesien_US
dc.relation.issn0264-410Xen_US
dc.description.volume40en_US
dc.description.issue16en_US
dc.description.startpage2409en_US
dc.description.endpage2419en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ2-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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