Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12389
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dc.contributor.authorIşık, Esinen_US
dc.contributor.authorBalkan, Çiğdemen_US
dc.contributor.authorKarl, Vivienen_US
dc.contributor.authorKarakaya, Hüseyin Çağlaren_US
dc.contributor.authorHua, Sansanen_US
dc.contributor.authorRauch, Sebastienen_US
dc.contributor.authorTamás, Markus Jen_US
dc.contributor.authorKoç, Ahmeten_US
dc.date.accessioned2022-08-16T08:26:29Z-
dc.date.available2022-08-16T08:26:29Z-
dc.date.issued2022-06-
dc.identifier.urihttps://doi.org/10.1002/mbo3.1284-
dc.identifier.urihttps://hdl.handle.net/11147/12389-
dc.description.abstractArsenic is a toxic metalloid that affects human health by causing numerous diseases and by being used in the treatment of acute promyelocytic leukemia. Saccharomyces cerevisiae (budding yeast) has been extensively utilized to elucidate the molecular mechanisms underlying arsenic toxicity and resistance in eukaryotes. In this study, we applied a genomic DNA overexpression strategy to identify yeast genes that provide arsenic resistance in wild-type and arsenic-sensitive S. cerevisiae cells. In addition to known arsenic-related genes, our genetic screen revealed novel genes, including PHO86, VBA3, UGP1, and TUL1, whose overexpression conferred resistance. To gain insights into possible resistance mechanisms, we addressed the contribution of these genes to cell growth, intracellular arsenic, and protein aggregation during arsenate exposure. Overexpression of PHO86 resulted in higher cellular arsenic levels but no additional effect on protein aggregation, indicating that these cells efficiently protect their intracellular environment. VBA3 overexpression caused resistance despite higher intracellular arsenic and protein aggregation levels. Overexpression of UGP1 led to lower intracellular arsenic and protein aggregation levels while TUL1 overexpression had no impact on intracellular arsenic or protein aggregation levels. Thus, the identified genes appear to confer arsenic resistance through distinct mechanisms but the molecular details remain to be elucidated.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofMicrobiologyOpenen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectArsenicen_US
dc.subjectYeasten_US
dc.subjectProtein aggregateen_US
dc.titleIdentification of novel arsenic resistance genes in yeasten_US
dc.typeArticleen_US
dc.authorid0000-0003-2914-7594en_US
dc.authorid0000-0003-3484-2137en_US
dc.institutionauthorIşık, Esinen_US
dc.institutionauthorBalkan, Çiğdemen_US
dc.institutionauthorKarakaya, Hüseyin Çağlaren_US
dc.institutionauthorKoç, Ahmeten_US
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.wosWOS:000789144000001en_US
dc.identifier.scopus2-s2.0-85132876893en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/mbo3.1284-
dc.identifier.pmid35765185-
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.contributor.affiliationUniversity of Gothenburgen_US
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.contributor.affiliationUniversity of Gothenburgen_US
dc.contributor.affiliationChalmers University of Technologyen_US
dc.contributor.affiliationUniversity of Gothenburgen_US
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.relation.issn2045-8827en_US
dc.description.volume11en_US
dc.description.issue3en_US
dc.identifier.scopusqualityQ3-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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