Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12609
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dc.contributor.authorKüçüksolak, Melisen_US
dc.contributor.authorÜner, Göklemen_US
dc.contributor.authorBallar Kırmızıbayrak, Peteken_US
dc.contributor.authorBedir, Erdalen_US
dc.date.accessioned2022-11-21T06:59:12Z-
dc.date.available2022-11-21T06:59:12Z-
dc.date.issued2022-12-
dc.identifier.urihttps://doi.org/10.1038/s41598-022-22799-5-
dc.identifier.urihttps://hdl.handle.net/11147/12609-
dc.description.abstractCyclocephagenol (1), a novel cycloartane-type sapogenin with tetrahydropyran unit, is only encountered in Astragalus species. This rare sapogenin has never been a topic of biological activity or modification studies. The objectives of this study were; (i) to perform microbial transformation studies on cyclocephagenol (1) using Astragalus endophyte, Alternaria eureka 1E1BL1, followed by isolation and structural characterization of the metabolites; (ii) to investigate neuroprotective activities of the metabolites; (iii) to understand structure–activity relationships towards neuroprotection. The microbial transformation of cyclocephagenol (1) using Alternaria eureka resulted in the production of twenty-one (2–22) previously undescribed metabolites. Oxidation, monooxygenation, dehydration, methyl migration, epoxidation, and ring expansion reactions were observed on the triterpenoid skeleton. Structures of the compounds were established by 1D-, 2D-NMR, and HR-MS analyses. The neuroprotective activities of metabolites and parent compound (1) were evaluated against H2O2-induced cell injury. The structure–activity relationship (SAR) was established, and the results revealed that 1 and several other metabolites had potent neuroprotective activity. Further studies revealed that selected compounds reduced the amount of ROS and preserved the integrity of the mitochondrial membrane. This is the first report of microbial transformation of cyclocephagenol.en_US
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.relation.ispartofScientific Reportsen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectCyclocephagenolen_US
dc.subjectBiotransformationen_US
dc.titleNeuroprotective metabolites via fungal biotransformation of a novel sapogenin, cyclocephagenolen_US
dc.typeArticleen_US
dc.authorid0000-0003-1619-4850en_US
dc.authorid0000-0003-4970-4837en_US
dc.authorid0000-0003-1262-063Xen_US
dc.institutionauthorKüçüksolak, Melisen_US
dc.institutionauthorÜner, Göklemen_US
dc.institutionauthorBedir, Erdalen_US
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.wosWOS:000878306800009en_US
dc.identifier.scopus2-s2.0-85141094140en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1038/s41598-022-22799-5-
dc.identifier.pmid36323752-
dc.relation.issn2045-2322en_US
dc.description.volume12en_US
dc.description.issue1en_US
dc.identifier.scopusqualityQ1-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
crisitem.author.dept03.01. Department of Bioengineering-
crisitem.author.dept01. Izmir Institute of Technology-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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