Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12662
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dc.contributor.authorAyaz Güner, Şerifetr
dc.contributor.authorAcar, Mustafa Buraktr
dc.contributor.authorBoyvat, Dudutr
dc.contributor.authorGüner, Hüseyintr
dc.contributor.authorBozalan, Habibetr
dc.contributor.authorGüzel, Melistr
dc.contributor.authorYıldır, Selin Kübratr
dc.contributor.authorAltınsoy, Nilaytr
dc.contributor.authorFındık, Fatmatr
dc.contributor.authorKarakükçü, Musatr
dc.contributor.authorÖzcan, Servettr
dc.date.accessioned2022-12-09T12:45:22Z-
dc.date.available2022-12-09T12:45:22Z-
dc.date.issued2022-
dc.identifier.urihttps://doi.org/10.1016/j.xpro.2022.101863-
dc.identifier.urihttps://hdl.handle.net/11147/12662-
dc.description.abstractAnalysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSTAR Protocolsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCell biologyen_US
dc.subjectCell isolationen_US
dc.subjectMass spectrometryen_US
dc.subjectProtein biochemistryen_US
dc.subjectProteomicsen_US
dc.titleProtocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cellsen_US
dc.typeArticleen_US
dc.authorid0000-0002-1052-0961en_US
dc.institutionauthorAyaz Güner, Şerifetr
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.wosWOS:001059416400008en_US
dc.identifier.scopus2-s2.0-85142326332en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr
dc.identifier.doi10.1016/j.xpro.2022.101863-
dc.relation.issn2666-1667en_US
dc.description.volume3en_US
dc.description.issue4en_US
dc.identifier.scopusqualityQ2-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairetypeArticle-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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