Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12663
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAcar, Mustafa Buraktr
dc.contributor.authorAyaz Güner, Şerifetr
dc.contributor.authorGüner, Hüseyintr
dc.contributor.authorDinç, Gökçentr
dc.contributor.authorUlu Kılıç, Ayşegültr
dc.contributor.authorDoğanay, Mehmettr
dc.contributor.authorÖzcan, Servettr
dc.date.accessioned2022-12-09T13:37:38Z-
dc.date.available2022-12-09T13:37:38Z-
dc.date.issued2022-11-
dc.identifier.urihttps://doi.org/10.3389/fimmu.2022.1001633-
dc.identifier.urihttps://hdl.handle.net/11147/12663-
dc.description.abstractBackground: Acinetobacter baumannii is one of the most life-threatening multidrug-resistant pathogens worldwide. Currently, 50%–70% of clinical isolates of A. baumannii are extensively drug-resistant, and available antibiotic options against A. baumannii infections are limited. There is still a need to discover specific de facto bacterial antigenic proteins that could be effective vaccine candidates in human infection. With the growth of research in recent years, several candidate molecules have been identified for vaccine development. So far, no public health authorities have approved vaccines against A. baumannii. Methods: This study aimed to identify immunodominant vaccine candidate proteins that can be immunoprecipitated specifically with patients’ IgGs, relying on the hypothesis that the infected person’s IgGs can capture immunodominant bacterial proteins. Herein, the outer-membrane and secreted proteins of sensitive and drug-resistant A. baumannii were captured using IgGs obtained from patient and healthy control sera and identified by Liquid Chromatography- Tandem Mass Spectrometry (LC-MS/MS) analysis. Results: Using the subtractive proteomic approach, we determined 34 unique proteins captured only in drug-resistant A. baumannii strain via patient sera. After extensively evaluating the predicted epitope regions, solubility, transverse membrane characteristics, and structural properties, we selected several notable vaccine candidates. Conclusion: We identified vaccine candidate proteins that triggered a de facto response of the human immune system against the antibiotic-resistant A. baumannii. Precipitation of bacterial proteins via patient immunoglobulins was a novel approach to identifying the proteins that could trigger a response in the patient immune system.en_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Immunologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectImmunodominanten_US
dc.subjectImmunoprecipitationen_US
dc.subjectProteomicsen_US
dc.subjectVaccine candidateen_US
dc.titleA subtractive proteomics approach for the identification of immunodominant Acinetobacter baumannii vaccine candidate proteinsen_US
dc.typeArticleen_US
dc.authorid0000-0002-1052-0961en_US
dc.institutionauthorAyaz Güner, Şerifetr
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.wosWOS:000889199500001en_US
dc.identifier.scopus2-s2.0-85142647917en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr
dc.identifier.doi10.3389/fimmu.2022.1001633-
dc.identifier.pmid36439128-
dc.relation.issn1664-3224en_US
dc.description.volume13en_US
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
fimmu-13-1001633.pdfArticle File8.38 MBAdobe PDFView/Open
Show simple item record



CORE Recommender

Page view(s)

102
checked on Apr 8, 2024

Download(s)

36
checked on Apr 8, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.