Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/13244
Title: Diagnosis of ruminant viral diseases with loop-mediated isothermal amplification
Authors: Ayaz Kök, Sanem
Üstün, Selcen
Taşkent, Hümeyra
Keywords: Infectious diseases
Isothermal nucleic acid amplification
Pen-side testing
Veterinary diagnostics
Issue Date: 2023
Publisher: Springer
Abstract: Infectious diseases in livestock industry are major problems for animal health, food safety, and the economy. Zoonotic diseases from farm animals are significant threat to human population as well. These are notifiable diseases listed by the World Organization for Animal Health (OIE). Rapid diagnostic methods can help keep infectious diseases under control in herds. Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid amplification method that is studied widely for detection of many infectious diseases in the field. LAMP allows biosensing of target DNA or RNA under isothermal conditions with high specificity in a short period of time. An untrained user can analyze results based on color change or turbidity. Here we review LAMP assays to diagnose OIE notifiable ruminant viral diseases in literature highlighting properties of LAMP method considering what is expected from an efficient, field usable diagnostic test.
Description: Authors wish to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) for funding (2210597) for S.A.K. and H.T.S. We would also like to thank Dr. Ahmet Sait from Pendik Veterinary Control Institute, Istanbul, Turkiye for critically reading the manuscript and for helpful discussion.
URI: https://doi.org/10.1007/s12033-023-00674-6
https://hdl.handle.net/11147/13244
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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