Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/13770
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dc.contributor.authorTamburaci, Sedef-
dc.contributor.authorPerpelek, Merve-
dc.contributor.authorAydemir, Selma-
dc.contributor.authorBaykara, Basak-
dc.contributor.authorHavitcioğlu, Hasan-
dc.contributor.authorTihminlioğlu, Funda-
dc.date.accessioned2023-10-03T07:15:29Z-
dc.date.available2023-10-03T07:15:29Z-
dc.date.issued2023-
dc.identifier.issn2576-6422-
dc.identifier.urihttps://doi.org/10.1021/acsabm.2c010501504-
dc.identifier.urihttps://hdl.handle.net/11147/13770-
dc.description.abstractRegeneration of osteochondral tissue with its layered complex structure and limited self-repair capacity has come into prominence as an application area for biomaterial design. Thus, literature studies have aimed to design multilayered scaffolds using natural polymers to mimic its unique structure. In this study, fabricated scaffolds are composed of transition layers both chemically and morphologically to mimic the gradient structure of osteochondral tissue. The aim of this study is to produce gradient chitosan (CHI) scaffolds with bioactive snail (Helix aspersa) mucus (M) and slime (S) extract and investigate the structures regarding their physicochemical, mechanical, and morphological characteristics as well as in vitro cytocompatibility and bioactivity. Gradient scaffolds (CHI-M and CHI-S) were fabricated via a layer-by-layer freezing and lyophilization technique. Highly porous and continuous 3D structures were obtained and observed with SEM analysis. In addition, scaffolds were physically characterized with water uptake test, micro-CT, mechanical analysis (compression tests), and XRD analysis. In vitro bioactivity of scaffolds was investigated by co-culturing Saos-2 and SW1353 cells on each compartment of gradient scaffolds. Osteogenic activity of Saos-2 cells on extract loaded gradient scaffolds was investigated in terms of ALP secretion, osteocalcin (OC) production, and biomineralization. Chondrogenic bioactivity of SW1353 cells was investigated regarding COMP and GAG production and observed with Alcian Blue staining. Both mucus and slime incorporation in the chitosan matrix increased the osteogenic differentiation of Saos-2 and SW1353 cells in comparison to the pristine matrix. In addition, histological and immunohistological staining was performed to investigate ECM formation on gradient scaffolds. Both characterization and in vitro bioactivity results indicated that CHI-M and CHI-S scaffolds show potential for osteochondral tissue regeneration, mimicking the structure as well as enhancing physical characteristics and bioactivity.en_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Applied Bio Materialsen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHelix aspersaen_US
dc.subjectgradient scaffolden_US
dc.subjectosteochondralen_US
dc.subjectmucusen_US
dc.subjectslimeen_US
dc.subjectENGINEERING APPLICATIONSen_US
dc.subjectFIBROIN SCAFFOLDen_US
dc.subjectSNAIL MUCUSen_US
dc.subjectDIFFERENTIATIONen_US
dc.subjectCONSTRUCTSen_US
dc.subjectCELLSen_US
dc.titleFabrication of Helix aspersa Extract Loaded Gradient Scaffold with an Integrated Architecture for Osteochondral Tissue Regeneration: Morphology, Structure, and In Vitro Bioactivity [1]en_US
dc.typeArticleen_US
dc.authoridBaykara, Basak/0000-0002-4178-2235-
dc.authoridTIHMINLIOGLU, Funda/0000-0002-3715-8253-
dc.authoridHavitcioglu, Hasan/0000-0001-8169-3539-
dc.authoridTAMBURACI, SEDEF/0000-0003-3234-226X-
dc.institutionauthor-
dc.departmentİzmir Institute of Technologyen_US
dc.identifier.volume6en_US
dc.identifier.issue4en_US
dc.identifier.startpage1504en_US
dc.identifier.endpage1514en_US
dc.identifier.wosWOS:000972554300001en_US
dc.identifier.scopus2-s2.0-85151909799en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1021/acsabm.2c010501504-
dc.identifier.pmid37009717en_US
dc.authorwosidBaykara, Basak/AAC-6504-2019-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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