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https://hdl.handle.net/11147/14379
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kartal, Rumeysa Bilginer | - |
dc.contributor.author | Yildiz, Ahu Arslan | - |
dc.date.accessioned | 2024-05-05T14:57:07Z | - |
dc.date.available | 2024-05-05T14:57:07Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 2470-1343 | - |
dc.identifier.uri | https://doi.org/10.1021/acsomega.3c08962 | - |
dc.identifier.uri | https://hdl.handle.net/11147/14379 | - |
dc.description | Arslan Yildiz, Ahu/0000-0003-0348-0575 | en_US |
dc.description.abstract | Magnetic levitation (MagLev) is a powerful and versatile technique that can sort objects based on their density differences. This paper reports the sorting of SH-SY5Y cells for neuronal differentiation by the MagLev technique. Herein, SH-SY5Y cells were differentiated with retinoic acid (RA) and brain-derived neurotrophic factor (BDNF). Neuronal differentiation was confirmed by neurite extension measurement and the immunostaining assay. Neurites reached the maximum length on day 9 after sequential treatment with RA-BDNF. Neuronal marker expression of un-/differentiated cells was investigated by beta-III tubulin and neuronal nuclei (NeuN) and differentiated cells exhibited a higher fluorescence intensity compared to un-/differentiated cells. MagLev results revealed that the density of differentiated SH-SY5Y cells gradually increased from 1.04 to 1.06 g/mL, while it remained stable at 1.05 g/mL for un-/differentiated cells. These findings signified that cell density would be a potent indicator of neuronal differentiation. Overall, it was shown that MagLev methodology can provide rapid, label-free, and easy sorting to analyze the differentiation of cells at a single-cell level. | en_US |
dc.description.sponsorship | , Izmir Y?ksek Teknoloji Enstit?s? [2022IYTE-1-0058]; IZTECH-Scientific Research Project (IYTE BAP grant); Izmir Institute of Technology, Biotechnology and Bioengineering Research and Application Centre (IZTECH-BIOMER) [TUBA-GEBIP 2019]; Turkish Academy of Science; TUBITAK 2211-A National Graduate Scholarship program | en_US |
dc.description.sponsorship | This study was supported by IZTECH-Scientific Research Project (IYTE BAP grant, 2022IYTE-1-0058). The authors acknowledge Izmir Institute of Technology, Biotechnology and Bioengineering Research and Application Centre (IZTECH-BIOMER), and Centre for Materials Research (IZTECH-MAM) for the instrumental facilities provided to accomplish this work. Ahu Arslan Yildiz gratefully acknowledges the Turkish Academy of Science (TUBA-GEBIP 2019). Rumeysa Bilginer Kartal gratefully acknowledges TUBITAK 2211-A National Graduate Scholarship program. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Amer Chemical Soc | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | [No Keyword Available] | en_US |
dc.title | Exploring Neuronal Differentiation Profiles in SH-SY5Y Cells through Magnetic Levitation Analysis | en_US |
dc.type | Article | en_US |
dc.authorid | Arslan Yildiz, Ahu/0000-0003-0348-0575 | - |
dc.department | Izmir Institute of Technology | en_US |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 13 | en_US |
dc.identifier.startpage | 14955 | en_US |
dc.identifier.endpage | 14962 | en_US |
dc.identifier.wos | WOS:001189955800001 | - |
dc.identifier.scopus | 2-s2.0-85188534581 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1021/acsomega.3c08962 | - |
dc.identifier.pmid | 38585102 | - |
dc.authorscopusid | 58952676600 | - |
dc.authorscopusid | 57217604248 | - |
dc.identifier.wosquality | Q2 | - |
dc.identifier.scopusquality | Q2 | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | 03.01. Department of Bioengineering | - |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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