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Title: Expression of Vitreoscilla hemoglobin in Gordonia amarae enhances biosurfactant production
Authors: Doğan, İlhan
Pagilla, Krishna R.
Webster, Dale A.
Stark, Benjamin C.
Keywords: Bioremediation
Biosurfactant production
Gordonia amarae
Trehalose lipid production
Vitreoscilla hemoglobin
Issue Date: Aug-2006
Publisher: Springer Verlag
Source: Doğan, İ., Pagilla, K. R., Webster, D. A., and Stark, B. C. (2006). Expression of Vitreoscilla hemoglobin in Gordonia amarae enhances biosurfactant production. Journal of Industrial Microbiology and Biotechnology, 33(8), 693-700. doi:10.1007/s10295-006-0097-0
Abstract: The gene (vgb) encoding Vitreoscilla (bacterial) hemoglobin (VHb) was electroporated into Gordonia amarae, where it was stably maintained, and expressed at about 4 nmol VHb g -1 of cells. The maximum cell mass (OD 600) of vgb-bearing G. amarae was greater than that of untransformed G. amarae for a variety of media and aeration conditions (2.8-fold under normal aeration and 3.4-fold under limited aeration in rich medium, and 3.5-fold under normal aeration and 3.2-fold under limited aeration in mineral salts medium). The maximum level of trehalose lipid from cultures grown in rich medium plus hexadecane was also increased for the recombinant strain, by 4.0-fold in broth and 1.8-fold in cells under normal aeration and 2.1-fold in broth and 1.4-fold in cells under limited aeration. Maximum overall biosurfactant production was also increased in the engineered strain, by 1.4-fold and 2.4-fold for limited and normal aeration, respectively. The engineered strain may be an improved source for producing purified biosurfactant or an aid to microorganisms bioremediating sparingly soluble contaminants in situ.
ISSN: 1367-5435
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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