Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2537
Title: Identification of a novel system for boron transport: Atr1 is a main boron exporter in yeast
Authors: Kaya, Alaattin
Karakaya, Hüseyin Çağlar
Fomenko, Dmitri E.
Gladyshev, Vadim N.
Koç, Ahmet
Keywords: Boron
Yeast
ATR1 gene
Saccharomyces cerevisiae
Carboxy terminal sequence
Gene overexpression
Issue Date: Jul-2009
Publisher: American Society for Microbiology
Source: Kaya, A., Karakaya, H. Ç., Fomenko, D. E., Gladyshev, V. N., and Koc, A.(2009). Identification of a novel system for boron transport: Atr1 is a main boron exporter in yeast. Molecular and Cellular Biology, 29(13), 3665-3674. doi:10.1128/MCB.01646-08
Abstract: Boron is a micronutrient in plants and animals, but its specific roles in cellular processes are not known. To understand boron transport and functions, we screened a yeast genomic DNA library for genes that confer resistance to the element in Saccharomyces cerevisiae. Thirty boron-resistant transformants were isolated, and they all contained the ATR1 (YML116w) gene. Atr1 is a multidrug resistance transport protein belonging to the major facilitator superfamily. C-terminal green fluorescent protein-tagged Atr1 localized to the cell membrane and vacuole, and ATR1 gene expression was upregulated by boron and several stress conditions. We found that atr1△ mutants were highly sensitive to boron treatment, whereas cells overexpressing ATR1 were boron resistant. In addition, atr1△ cells accumulated boron, whereas ATR1-overexpressing cells had low intracellular levels of the element. Furthermore, atr1△ cells showed stronger boron-dependent phenotypes than mutants deficient in genes previously reported to be implicated in boron metabolism. ATR1 is widely distributed in bacteria, archaea, and lower eukaryotes. Our data suggest that Atr1 functions as a boron efflux pump and is required for boron tolerance.
URI: http://dx.doi.org/10.1128/MCB.01646-08
http://hdl.handle.net/11147/2537
ISSN: 0270-7306
0270-7306
1098-5549
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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