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Title: Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes
Authors: Demir, Ayşe Banu
Koç, Ahmet
Keywords: Antioxidant genes
Chronological aging
Molecular damage
Oxidative stress
Issue Date: Sep-2010
Publisher: Elsevier Ltd.
Source: Demir, A. B., and Koç, A. (2010). Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes. Biochemical and Biophysical Research Communications, 400(1), 106-110. doi:10.1016/j.bbrc.2010.08.019
Abstract: The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: G. RX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1Δ, trx3Δ, grx5Δ, prx1Δ, mutants were sensitive to diamide, and ccs1Δ and sod2Δ were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content. © 2010 Elsevier Inc.
ISSN: 0006-291X
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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