Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2719
Title: Inhibition of catechol-O-methyltransferase (COMT) by some plant-derived alkaloids and phenolics
Authors: Yalçın, Dilek
Bayraktar, Oğuz
Keywords: Phenols
Alkaloids
COMT
Inhibition
Parkinson's disease
Issue Date: Jul-2010
Publisher: Elsevier Ltd.
Source: Yalçın, D., and Bayraktar, O. (2010). Inhibition of catechol-O-methyltransferase (COMT) by some plant-derived alkaloids and phenolics. Journal of Molecular Catalysis B: Enzymatic, 64(3-4), 162-166. doi:10.1016/j.molcatb.2009.04.014
Abstract: In this study, as an alternative to the medicines, natural compounds extracted from plant species (Peganum harmala, Cistus parviflorus and Vitex agnus-cactus) were investigated in order to inhibit the catechol-O-metyhltranferase (COMT) activity. In fluorometric enzyme assay, S-adenosylmethionine (SAM) and aesculetin (ES) were used as methyl donor and acceptor substrates, respectively. Their Km values were determined as 3.5 ± 0.3 μM and 6.4 ± 0.4 μM in absence of inhibitor. Inhibition performances of the plant-derived polyphenolics and alkaloids were determined. Inhibitory effect of alkaloids extracted from P. harmala seeds was found the highest among the plant extracts; however, it was lower than that of 3,5-DNC. In case of inhibition mechanism, mixed type inhibition was observed for alkaloid extract whereas uncompetitive inhibition was observed for 3,5-DNC. In case of polyphenolic extracts obtained from C. parviflorus and V. agnus-cactus leaves, mechanism were also explained as mixed type inhibition and their αKi values were calculated as 1.99 ± 0.35 μg/ml and 9.48 ± 0.58 μg/ml, respectively. © 2009 Elsevier B.V. All rights reserved.
URI: http://doi.org/10.1016/j.molcatb.2009.04.014
http://hdl.handle.net/11147/2719
ISSN: 1381-1177
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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