Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2757
Title: Label-free quantitation, an extension to 2DB
Authors: Allmer, Jens
Keywords: Spectral count
Software
Quantification
Label-free
Tandem mass spectrometry
Issue Date: Apr-2010
Publisher: Springer Verlag
Source: Allmer, J. (2010). Label-free quantitation, an extension to 2DB. Amino Acids, 38(4), 1075-1087. doi:10.1007/s00726-009-0317-9
Abstract: Determining the differential expression of proteins under different conditions is of major importance in proteomics. Since mass spectrometry-based proteomics is often used to quantify proteins, several labelling strategies have been developed. While these are generally more precise than label-free quantitation approaches, they imply specifically designed experiments which also require knowledge about peptides that are expected to be measured and need to be modified. We recently designed the 2DB database which aids storage, analysis, and publication of data from mass spectrometric experiments to identify proteins. This database can aid identifying peptides which can be used for quantitation. Here an extension to the database application, named MSMAG, is presented which allows for more detailed analysis of the distribution of peptides and their associated proteins over the fractions of an experiment. Furthermore, given several biological samples in the database, label-free quantitation can be performed. Thus, interesting proteins, which may warrant further investigation, can be identified en passant while performing high-throughput proteomics studies. © 2009 Springer-Verlag.
URI: http://doi.org/10.1007/s00726-009-0317-9
http://hdl.handle.net/11147/2757
ISSN: 0939-4451
0939-4451
1438-2199
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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