Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3033
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dc.contributor.advisorTarı, Cananen
dc.contributor.authorDoğan, Nergiz-
dc.date.accessioned2014-07-22T13:50:45Z-
dc.date.available2014-07-22T13:50:45Z-
dc.date.issued2008en
dc.identifier.urihttp://hdl.handle.net/11147/3033-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2008en
dc.descriptionIncludes bibliographical references (leaves: 57-60)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionx, 74 leavesen
dc.description.abstractCommercial preparations of pectinases derived from fungi are well known to have high biotechnological value in the industry. For this purpose, polymethylgalacturonase (PMG) and polygalacturonase (PG) were produced with high productivities by Aspergillus sojae ATCC 20235 by using low cost carbon (Maltrin) and nitrogen (Corn Steep Liquor, CSL) sources. There is no literature report to best of our knowledge on the fed-batch production, purification and characterization of polygalacturonase using this microorganism.In this study batch fermentation was carried out in order to obtain the crude PG and to establish a baseline for the forth coming fed-batch experiments. The crude PG was partially purified using three-phase partitioning as an emerging bioseparation technique and characterized with respect to its biochemical and thermal properties. These studies showed that this enzyme holds a great potential to be a good candidate for various industrial applications. To optimize fed-batch fermentation conditions, response surface methodology (RSM) was performed using face-centered central composite design. As a result, maximum PG activity (20.61 U/ml) and maximum biomass (34.23 g/l) were obtained at high maltrin (150 g/l) and high CSL (10 g/l) concentrations when the repeated feeding was done at 48th and 72nd hours. Maximum PMG activity (16.76 U/ml) was also achieved at higher maltrin and higher CSL concentrations at a feeding time of 72nd hours. Fed-batch fermentation has been successfully used to increase PG (33.74%) and PMG (23.96%) activities from Aspergillus sojae. Finally, agar diffusion method was adapted as a rapid method for the selection of high pectinase producer in the strain improvement study.en
dc.language.isoenen_US
dc.publisherIzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccTP248.27.F86 D65 2008en
dc.subject.lcshFungi--Biotechnologyen
dc.subject.lcshPolygalacturonaseen
dc.subject.lcshAspergillus--Biotechnologyen
dc.titleProduction of pectinase enzyme from aspergillus sojae batch and fed-batch systemsen_US
dc.typeMaster Thesisen_US
dc.institutionauthorDoğan, Nergiz-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
dc.relation.publicationcategoryTezen_US
item.fulltextWith Fulltext-
item.openairetypeMaster Thesis-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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