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Title: Isolation, expression, characterization of an a-L-arabinofuranosidase enzyme from Thermophilic Geobacillus sp.
Authors: Şanlı Mohamed, Gülşah
İlgü, Hüseyin
Issue Date: 2011
Publisher: Izmir Institute of Technology
Abstract: In our study, we have aimed first to isolate an a-L-arabinofuranosidase (ALAF) enzyme, 58.0 kDa, from a thermophilic organism; Thermophilic Geobacillus sp. by using molecular cloning techniques, then to characterize this enzyme via biochemical methods. Throughout the characterization studies, we have investigated the optimum conditions for the highest enzyme activity by means of pH and temperature by using pNP--L-arabinofuranoside as substrate. Also, effect of various metal ions, some specific chemicals and common organic solvents on enzyme activity was studied. Due to the fact that -L-arabinofuranosidases mainly hydrolyze -L-arabinofuranosyl residues of L-arabinose containing polysaccharides, enzyme activity towards sugar beet arabinan was also studied. Our enzyme exhibited activity in a broad pH range between pH 3.0-10.0 at 50°C and between 30-90°C in Na-acetate buffer pH 5.0. Optimum activity towards pNP--L-arabinofuranoside was obtained at pH 5.0 and at 70°C. Kinetic studies showed that our enzyme has a Km value as 0.19 mM and Vmax as 18.6 Abs/min/ml towards pNP--L-arabinofuranoside and Km value as 0.1 mM and Vmax as 8.1 Abs/min/ml towards sugar beet arabinan.
Description: Thesis (Master)--İzmir Institute of Technology, Chemistry, İzmir, 2011
Includes bibliographical references (leaves: 47-51)
Text in English; Abstract: Turkish and English
xii, 51 leaves
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

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