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dc.contributor.advisorKoç, Ahmeten
dc.contributor.authorEsmer, Işıl-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2011en
dc.descriptionIncludes bibliographical references (leaves: 25-32)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionx, 33 leavesen
dc.description.abstractAutophagy is defined as a catabolic bulk degradation pathway. In S.cerevisiae autophagy is defined as a single cell's adaptation to starvation. Ageing can be defined as a gradual and progressive deterioration of the health by the time. S.cerevisiae is one of the most important model organism in the field of ageing studies. It has been demonstrated that in different model organisms cellular ageing requires inhibiton of particular nutrient sensing pathways leads to extension or shorten replicative and chronological life span that would be linked by regulation of autophagic pathway. In our study, we screened 29 deletion mutants of autophagy genes in order to determine relation between autophagy and ageing. We found out that 6 mutants showed reduced replicative life span. Furthermore, possible roles of boric acid, as an autophagy inducer was elucidated on replicative and chronological life span. Boric acid did not incerease replicative or chronological life span, in oppose, it shortened life span in a dosedependent manner. Finally, to find out the link between autophagy and oxidative stress, we tested autophagy deficient cells for their hydrogen peroxide sensitivity. Absence of only five mutants rendered cells hydrogen peroxide sensitive. Overall, our results revealed that several mutants of autophagy related paths have shorter life spans compared to wild type cells and cells need to have an intact autophagy systems to benefit from life span extension.en
dc.publisherIzmir Institute of Technologyen
dc.publisherIzmir Institute of Technologyen_US
dc.subject.lcshAging--Genetic aspectsen
dc.subject.lcshAutophagic vacuolesen
dc.titleThe effects of autophagy genes on ageingen_US
dc.typeMaster Thesisen_US
dc.institutionauthorEsmer, Işıl-
dc.departmentThesis (Master)--İzmir Institute of Technology, Molecular Biology and Geneticsen_US
item.fulltextWith Fulltext-
item.openairetypeMaster Thesis-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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