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dc.contributor.advisorYemenicioğlu, Ahmeten
dc.contributor.authorArcan, İskender-
dc.descriptionThesis (Master)--İzmir Institute of Technology, Biotechnology and Bioengineering, İzmir, 2005en
dc.descriptionIncludes bibliographical references (leaves: 73-75)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxiv, 92 leavesen
dc.description.abstractIn this study, the radical scavenging and iron chelating capacity of proteins from heat treated (20 min at 90 oC) or thermally processed (20 min at 121 oC) chick-peas andkidney-beans were compared. Lyophilized crude protein extracts from chick-peas contained more protein (1.5-3 fold) and showed higher free radical scavenging (up to 2.3 fold) and iron binding capacity (up to 3 fold) than lyophilized crude protein extracts form kidney-beans. The thermal processing of chick-peas did not cause a significant change in the radical scavenging capacity of their lyophilized crude protein extracts, but improved the iron chelating capacity of these proteins almost 80 %. However, the thermal processing reduced both the radical scavenging and iron binding capacity of crude lyophilized proteins form kidney beans by 20-40 % and 60 %, respectively.Partial purification by ammonium sulfate precipitation or DEAE-cellulose chromatography increased the antioxidant capacity of thermally processed chick-pea proteins. The DEAE cellulose chromatography also showed the presence of 5 and 3 antioxidant protein fractions in heat treated and thermally processed chick-peas, respectively. Hot acidic hydrolysis at 80 oC for 30 min in presence of 1.5 M HCl increases the specific antioxidant activity of protein extracts, but causes the formation of undesired Maillard reaction products. Hot extraction at 85 oC for 30 min at pH 2.5 extracts the antioxidant proteins selectively, whereas 85 oC for 30 min at pH 9.5 extracts both antioxidant proteins and other proteins.en
dc.publisherIzmir Institute of Technologyen_US
dc.titleCharacterization and modification of antioxidant proteins from plat materialsen_US
dc.typeMaster Thesisen_US
dc.institutionauthorArcan, İskender-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
item.fulltextWith Fulltext-
item.openairetypeMaster Thesis-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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