Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3307
Title: Genetic characterization of cucumber mosaic virus (CMV) resistance in tomato and pepper
Authors: Doğanlar, Sami
Balcı, Evrim
Issue Date: 2005
Publisher: Izmir Institute of Technology
Abstract: In this study, tomato and pepper populations were phenotypically and genotypically characterized to identify cucumber mosaic virus (CMV) resistant lines and determine the genetic control of resistance. Populations of both crops and their resistant and susceptible parents were mechanically inoculated with CMV. Plants were evaluated visually and by enzyme-linked immunosorbent assay (ELISA). Since virus was detected only in inoculated leaves but was not usually present in uninoculated leaves of symptomless plants, CMV resistance appeared to be true resistance not immunity. According to phenotypic analysis of F2 tomato population, it was hypothesized that two dominant genes were controlling resistance as the plants fit a 9:7 (resistant:susceptible) segregation ratio as determined by Chi square goodness-of-fit analysis. In order to perform quantitiative trait locus (QTL) analysis, molecular markers were surveyed for polymorphism using the two parents of the tomato population, L. esculentum and L. hirsutum LA1223. According to QTL analysis (using 107 polymorphic markers), 11 genomic regions were linked to CMV resistance. For seven loci, resistance alleles were coming from the L. hirsutum parent as expected. However, for four loci, resistance was associated with alleles from the CMV susceptible parent, L. esculentum. These results show that the genetic potential for resistance cannot be determined by only looking at the phenotype of the two parents. As a result of this work, developing elite lines or transferring resistance genes into cultivated species by marker assisted selection will be easier.
Description: Thesis(Master)--İzmir Institute of Technology, Biology, İzmir, 2005
Includes bibliographical references (p.49-55)
Text in English; Abstract: Turkish and English
x, 55 leaves
URI: http://hdl.handle.net/11147/3307
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

Files in This Item:
File Description SizeFormat 
T000388.pdfMasterThesis2.55 MBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

Page view(s)

62
checked on Sep 26, 2022

Download(s)

32
checked on Sep 26, 2022

Google ScholarTM

Check


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.