Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3378
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dc.contributor.advisorYenidünya, A.Fazilen
dc.contributor.authorAltan, Asena-
dc.date.accessioned2014-07-22T13:51:24Z
dc.date.available2014-07-22T13:51:24Z
dc.date.issued2004en
dc.identifier.urihttp://hdl.handle.net/11147/3378
dc.descriptionThesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2004en
dc.descriptionIncludes bibliographical references (leaves: 65-74)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionx, 88 leavesen
dc.description.abstractLipases and pectinases are industrially important enzymes. These enzymes are produced by a variety of microorganisms. However there are few studies on the production of these enzymes by thermoacidophilic Bacillus species. The aim of this research was the isolation of extracellular lipase and pectinase producing thermoacidophilic Bacillus from olive oil mills and their identification by phenotypic tests, 16S-ITS rDNA RFLP and DNA sequencing. Eighty-six thermoacidophilic strains were isolated from olive, olive husk and soil contaminated with alpechin collected within different olive oil mills in Ayvalık. The strains were screened for the presence of 5 extracellular enzyme activities. These were lipase, pectinase, amylase, xylanase and cellulase. In total, 69 lipase (Tween 20 as subtrate), 32 pectinase and 68 amylase activities were detected. None of the isolates were able to produce xylanase or cellulase enzyme. All of the isolates were Gram(+) endospore forming rods, thus they were identified as Bacillus sp. Taq I was used for 16S-ITS rDNA based RFLP. The isolated strains were clustered into four groups by Taq I restriction profiles of 16S-ITS rDNA. One representative isolate among the members of each of the 16S-RFLP homology groups was chosen and used for 16S rRNA gene partial sequence analysis. Sequencing results were submitted to GenBank. So far the indicated accession numbers were obtained: AY601903 (isolate H 22 of G-3, 679 nucleotides), AY606276 (isolate S1 of G1, 330 nucleotides)en
dc.language.isoenen_US
dc.publisherIzmir Institute of Technologyen
dc.publisherIzmir Institute of Technologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccQR82.B3 .A46 2004en
dc.subject.lcshBacillus (Bacteria)en
dc.subject.lcshEnzymes--Industrial applicationsen
dc.subject.lcshPolygalacturonaseen
dc.subject.lcshLipaseen
dc.titleIsolation and molecular characterization of extracellular lipase and pectinase producing bacteria from olive oil millsen_US
dc.typeMaster Thesisen_US
dc.authoridTR115720
dc.institutionauthorAltan, Asena-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
dc.relation.publicationcategoryTezen_US
item.openairetypeMaster Thesis-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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