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dc.contributor.advisorAkgül, Bünyaminen
dc.contributor.authorDemir, Şeyda-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2012en
dc.descriptionIncludes bibliographical references (leaves: 20-22)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionix, 22 leavesen
dc.description.abstractMicroRNAs are non-coding 19-25nt long, small RNAs that regulate expression of about 30% of human genes by inhibiting mRNA translation or inducing its degradation. MicroRNAs play important role in cell growth, differentiation, apoptosis. miRNAs regulate apoptosis by targeting genes involved in apoptotic pathway as a pro or anti-apoptotic genes. This study has aimed to identify whether candidate miRNAs ( miR-17* and miR-425) have a regulatory role in camptothecin induced apoptosis or not in Human cells and Hela cells that derived from cervical cancer were used as a model cell line. These candidates were selected based on deep sequencing data that showed some miRNAs differentially expressed after camptothecin treatment as compared with non-treated control group. To show candidate miRNAs whether have a role or not in regulation of camptothecin induced apoptosis, first Hela cells were transfected with candidate miRNAs then candidate miRNA over-expressed cells were treated with camptothecin eventually level of apoptosis was measured by flow cytometry and the results were evaluated by comparing miRNA over-expressed cell group with un-transfected control group. Active caspase-3 level also was measured by using flow cytometry and the data showed miR-17* and miR-425 function as pro-apoptotic regulator in camptothecin induced apoptosis in Hela cells.en
dc.publisherIzmir Institute of Technologyen
dc.subject.lcshRNA editingen
dc.subject.lcshGenetic regulationen
dc.titleInvestigation of the functions of candidate miRNAs in camptothecin-induced apoptosis in human cellsen_US
dc.typeMaster Thesisen_US
dc.institutionauthorDemir, Şeyda-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen
item.openairetypeMaster Thesis-
item.fulltextWith Fulltext-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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