Please use this identifier to cite or link to this item:
Title: Production and isolation of fungal chitosan by submerged fermentation
Authors: Alper, Seda
Advisors: Harsa, Hayriye Şebnem
Publisher: Izmir Institute of Technology
Abstract: Chitosan is the N-deacetylated derivative of chitin which is the supporting material of crustaceans and insects. Chitosan together with chitin are recommended as suitable functional materials because of their excellent biocompatibility, biodegradability, non-toxicity and adsorption properties and can be used in agriculture, biotechnology and food industry. Although chitosan is produced by chemical deacetylation of chitin molecule, it is also a natural component of cell walls of fungi belonging to Zygomycetes and can be produced by extraction from fungus cell walls. Fungi are thus the promising alternative sources of chitosan. Fungi can be manipulated to give chitosan of more consistent and desired physico-chemical properties compared to chitosan obtained from crustacean sources. In this study, Absidia spp, Aspergillus niger, Rhizopus arrhizus, Cunnighamella elegans, and Mucor rouxii were examined for biomass growth. At first, all five species were grown on synthetic medium at 28 C, 180 rpm in shake-flask incubator. Mucorrouxii which gave the maximum biomass concentration was also grown on molasses. The maximum biomass concentration of Mucor rouxii was found to be higher than that of synthetic medium. The best growth conditions obtained were 4% sucrose, 0.2% yeast extract, 1% peptone and 106 spores in 40 ml. The mycelia harvested at late exponential phase was treated with alkali to remove proteins and chitosan was extracted from cell wall by using acetic acid. The yield of extractable chitosan obtained from cell wall of Mucor rouxii was 2500 mg / l and it is almost 20 % of biomass and approximately 35 % of alkali insoluble fraction.
Description: Thesis (Master)--İzmir Institute of Technology, Biotechnology and Bioengineering, İzmir, 2003
Includes bibliographical references (leaves: 50-52)
Text in English;Abstract: Turkish and English
xi, 74 leaves
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

Files in This Item:
File Description SizeFormat 
T000249.pdfMasterThesis5.59 MBAdobe PDFThumbnail
Show full item record

CORE Recommender

Page view(s)

checked on May 27, 2024


checked on May 27, 2024

Google ScholarTM


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.