Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3699
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dc.contributor.advisorBaran, Yusufen
dc.contributor.authorBaşsoy, Esen Yonca-
dc.date.accessioned2014-07-22T13:52:10Z-
dc.date.available2014-07-22T13:52:10Z-
dc.date.issued2009en
dc.identifier.urihttp://hdl.handle.net/11147/3699-
dc.descriptionThesis (Master)--İzmir Institute of Technology, Biotechnology and Bioengineering, İzmir, 2009en
dc.descriptionIncludes bibliographical references (leaves: 47-54)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxi, 54 leavesen
dc.description.abstractPatients diagnosed with prostate cancer initially respond to androgen ablation therapy with tumor cells undergoing apoptosis, but then the patients relapse in time and develop metastatic, androgen independent prostate cancer. Docetaxel has been widely used for treatment of patients with advanced metastatic prostate cancer. The sphingolipid, ceramide, is a lipid second messenger that mediates a lot of functions as regulation of cell growth, proliferation, differentiation, senescence and apoptotic responses in various cancer cells. The enzyme, glucosylceramide synthase (GCS) is responsible for bioactivation of the proapoptotic mediator ceramide to antiapoptotic glucosylceramide. Likewise, sphingosine kinase-1 (SK-1) transforms apoptotic ceramide to antiapoptotic sphingosine 1-phosphate. Emerging results indicate that GCS and SK-1 are overexpressed in resistant cancer cell lines and cancerous tissue samples of patients. Moreover apoptosis and inhibition of cell proliferation and survival are induced by intracellular ceramide levels including enhancement in de novo ceramide production, exogenous delivery of cell permeable ceramide and inhibition of ceramide metabolism by affecting GCS and SK-1. In this study, we applied exogenous ceramide and inhibitors of GCS and SK-1 in combination with docetaxel for sensitizing androgen independent prostate cancer cells to chemotherapy and provide their effectively utilization with minimizing side effects of the drugs. The de novo generation of ceramide is regulated by the genes (LASS1-6) in mammalian cells. Therefore in this study, we examined the possible roles of the ceramide/S1P and ceramide/GS by examining expression levels of GCS, SK-1 and LASS1,2,4,5,6 which can play important roles to overcome androgen independent.en
dc.language.isoenen_US
dc.publisherIzmir Institute of Technologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccRC280.P7 .B317 2009en
dc.subject.lcshProstate--Canceren
dc.subject.lcshCeramidesen
dc.subject.lcshDocetaxelen
dc.subject.lcshSphingolipidsen
dc.titleThe involvement of ceramide metabolizing genes and their products in docetaxel induced apoptosis in human prostate cancer cellsen_US
dc.typeMaster Thesisen_US
dc.institutionauthorBaşsoy, Esen Yonca-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
dc.relation.publicationcategoryTezen_US
item.openairetypeMaster Thesis-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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