Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3810
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dc.contributor.advisorKarakaya, Hüseyin Çağlaren
dc.contributor.authorAkıncı, Ersin-
dc.date.accessioned2014-07-22T13:52:25Z-
dc.date.available2014-07-22T13:52:25Z-
dc.date.issued2007en
dc.identifier.urihttp://hdl.handle.net/11147/3810-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2007en
dc.descriptionIncludes bibliographical references (leaves: 39-43)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxi, 75 leavesen
dc.description.abstractBoron, is a microelement that plays a role in plant development. In contrast, excess amount of boron is toxic for plants. Turkey is the second country that has the largest boron reserve in the world, thus boron is one of the major problems in agriculture in Turkey. Barley (Hordeum vulgare) is the second widely produced cereal after wheat. Because barley is used in human diet, animal feeding and beer industry it is an economically valuable crop. There are ten different barley varieties in Turkey and these varieties show different genetic variations against boron toxicity. In this study, Hamidiye (boron sensitive) and Anadolu (boron tolerant) varieties were used to identify genes responsible for boron tolerance. RT-PCR and mRNA Differential Display techniques were used from root and leaf samples of Hamidiye and Anadolu varieties grown in laboratory with or without boron conditions. Eight differentially expressed genes identified by using mRNA Differential Display technique. Sequence of these genes gave homology to an eukaryotic translation initiation factor in Arabidopsis thaliana, a chlorophyll a/b binding protein precursor in Triticum aestivum, an elongation factor in Oryza sativa, short-chain dehydrogenase/reductase family protein in Arabidopsis thaliana, a thioredoxin h isoform in Triticum aestivum, a shaggy-like kinase protein in Triticum aestivum, chloroplast genome in Hordeum vulgare, a hypothetical protein in Arabidopsis thaliana. Expression level of six of forty three antiporter genes showed differences between Anadolu and Hamidiye cultivars in Real Time PCR.en
dc.language.isoenen_US
dc.publisherIzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccQH450.25 A31 2007en
dc.subject.lcshRNA editingen
dc.subject.lcshPlants, Effect of boron onen
dc.subject.lcshBarley--Geneticsen
dc.titleIdentification and characterization of boron tolerant genes in Hordeum vulgare (Barley) by using mRNA differential display and RT-PCR techniquesen_US
dc.typeMaster Thesisen_US
dc.institutionauthorAkıncı, Ersin-
dc.departmentThesis (Master)--İzmir Institute of Technology, Molecular Biology and Geneticsen_US
dc.relation.publicationcategoryTezen_US
item.openairetypeMaster Thesis-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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