Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4051
Title: Cloning of polysome-associated small RNAs in Drosophila melanogaster embryos
Authors: Akgül, Bünyamin
Yiğit, Hatice
Issue Date: 2009
Publisher: Izmir Institute of Technology
Abstract: Genome-encoded regulatory small RNAs are classified into 3 groups; microRNAs (miRNAs), endogeneous small interfering RNAs (endo siRNAs) and piwi interacting RNAs (piRNAs). miRNAs, 17-21 nucleotide in size, are involved in posttranscriptional gene regulation via precise or imprecise base pairing with target mRNAs resulting in either target mRNA degradation or translational inhibition. Endo siRNAs ,on the other hand, may function transposon regulation but their precise regulatory function and mechanism have not been elucidated yet. piRNAs are mainly involved in transposon silencing in spermatogenesis. Despite their discovery, biological roles and modes of functions of small RNAs remain to be elucidated. The aim of this thesis was to identify polysome-associated small RNAs in Drosophila melanogaster embryos by deep sequencing and investigate their role in translational regulation. Deep sequencing and microarray results determined stage and fraction specific distribution of genome encoded small RNAs. Surprisingly, the results implied that mRNAs may be posttranscriptionally regulated by antisense transcripts in polysome.
Description: Thesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2009
Includes bibliographical references (leaves: 29-33)
Text in English; Abstract: Turkish and English
x, 33 leaves
URI: http://hdl.handle.net/11147/4051
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

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