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dc.contributor.advisorBulmuş Zareie, Esma Volga-
dc.contributor.authorUğur, Deniz-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2014en_US
dc.descriptionIncludes bibliographical references (leaves: 53-58)en_US
dc.descriptionText in English; Abstract: Turkish and Englishen_US
dc.descriptionx, 58 leavesen_US
dc.description.abstractIn this study, a higly cationic biosynthetic polymer, poly(Arginine Methyl Ester Methacrylamide) (p(AMME)) has been designed as a potential component of intracellular delivery systems for biological macromolecular therapeutics such as nucleic acids. Accordingly, an arginine derivative monomer; Arginine Methyl Ester Methacrylamide (AMME) was synthesized by the reaction of an active ester monomer, pentafluorophenylmethacrylate (PFMA) and the L-arginine methyl ester hydrochloride (AME) in the presence of excess triethyl amine. AMME was then polymerized via both conventional free radical polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymers p(AMME) were characterized via nuclear magnetic resonance and gel permeation chromatography techniques. The conventional free radical polymerization experiments yielded polymers with uncontrolled molecular weights and wide molecular weight distribution, whereas RAFT polymerizations performed both in aqueous solution and organic solvent yielded polymers with controlled molecular weights and narrow molecular weight distributions. The RAFT polymerization kinetic experiments showed the RAFT-controlled character of AMME polymerization in the presence of 4-cyano-4-(ethylthiocarbonylthioylthio) sulfanylpentanoic acid (ECT) as a RAFT agent. The cytotoxicity of P(AMME) before and after aminolysis was determined via MTT assay using A549 human lung cancer cell line. P(AMME) before aminolysis of the RAFT end-group displayed dose-dependent toxicity after 24 hours incubation with cells. It was highly toxic to cells at 25 μM concentration, killing almost more than 60% of cells after 24 hours incubation. On the other hand, the aminolyzed polymer has no significant toxicity in the concentration range studied (upto 500 μM), which was comparable with octaarginine, a widely used transfection agent. In conclusion, well-defined arginine-polymers synthesized in this study show potential for further investigations as potential components of intracellular delivery systems for therapeutics.en_US
dc.publisherIzmir Institute of Technologyen_US
dc.subjectRadical polymerizationen_US
dc.titleSynthesis and raft polymerization of arginine containing monomer to investigate the cell membrane translocationen_US
dc.title.alternativeHücre membranından translokasyonu incelemek için arjinin içeren monomerin sentezi ve RAFT polimerizasyonuen_US
dc.typeMaster Thesisen_US
dc.institutionauthorUğur, Deniz-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
item.openairetypeMaster Thesis-
item.fulltextWith Fulltext-
item.languageiso639-1en- Izmir Institute of Technology-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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