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dc.contributor.advisorPesen Okvur, Devrimen_US
dc.contributor.authorHorzum, Utku-
dc.identifier.citationHorzum, U. (2014). Cell adhesion on nanomater scale fibronectin patterns: A comparision of breast cancer cells and normal breast epithelial cells. Unpublished master's thesis, İzmir Institute of Technology, İzmir, Turkeyen_US
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2014en_US
dc.descriptionIncludes bibliographical references (leaves: 66-72)en_US
dc.descriptionText in English; Abstract: Turkish and Englishen_US
dc.descriptionxii, 72 leavesen_US
dc.descriptionFull text release delayed at author's request until 2018.01.26en_US
dc.description.abstractCell adhesion to extracellular matrix is an important process for both health and disease states. Surface protein patterns are topographically flat, and do not introduce other chemical, topographical or rigidity related functionality and, more importantly, that mimic the organization of the in vivo extracellular matrix are desirable. Previous work showed that vinculin and cytoskeletal organization are modulated by the size and shape of surface nanopatterns. However, a comparative and quantitative analysis on normal and cancerous cell morphology and focal adhesions as a function of micrometer scale spacings of protein nanopatterns was absent. Here, electron beam lithography was used to pattern fibronectin (FN) nanodots with micrometer scale spacings on a K-casein background (single active) on indium tin oxide (ITO) coated glass which, unlike silicon, is transparent and thus suitable for many light microscopy techniques. Exposure times were significantly reduced using the line exposure mode with micrometer scale step sizes. Micrometer scale spacings of 2, 4, 8 microns and gradients between FN nanodots modulated cell adhesion for both breast cancer and normal mammary epithelial cells, through modification of cell area, cell symmetry, actin organization, focal adhesion number, size and circularity under both static and flow conditions. Overall, cell behavior was shown to shift at the apparent threshold of 4 μm spacing. Results showed that there were significant differences in terms of cell adhesion between breast cancer and normal mammary epithelial cells: Breast cancer cells exhibited a more dynamic and flexible adhesion profile than normal mammary epithelial cells.en_US
dc.publisherIzmir Institute of Technologyen_US
dc.subject.lcshCell adhesion--Molecular aspectsen_US
dc.subject.lcshCancer cellsen_US
dc.titleCell adhesion on nanomater scale fibronectin patterns: A comparision of breast cancer cells and normal breast epithelial cellsen_US
dc.title.alternativeNanometre ölçeğinde fibronektin desenleri üzerinde hücre yapışması: Meme kanseri hücreleri ve normal meme epitel hücrelerinin karşılaştırılmasıen_US
dc.typeMaster Thesisen_US
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
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item.openairetypeMaster Thesis-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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