Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4559
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dc.contributor.advisorFrary, Anneen_US
dc.contributor.authorGöktay, Mehmet-
dc.date.accessioned2016-04-27T08:06:04Z-
dc.date.available2016-04-27T08:06:04Z-
dc.date.issued2015-12-
dc.identifier.citationGöktay, M. (2015). Characterization of world spinach genetic collection by using molecular markers. Unpublished master's thesis, İzmir Institute of Technology, İzmir, Turkeyen_US
dc.identifier.urihttp://hdl.handle.net/11147/4559-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics., Izmir, 2015en_US
dc.descriptionIncludes bibliographical references (leaves: 28-29)en_US
dc.descriptionText in English; Abstract: Turkish and Englishen_US
dc.descriptionix, 29 leavesen_US
dc.description.abstractSpinach (Spinacia oleracea L.) belongs to the Amaranthaceae family and is a popular and nutritious vegetable. It is believed that this green leafy plant originated from Persia which is now modern Iran and neighboring countries. In this study we screened 176 spinach world collection germplasm accessions with 15 known SSR markers. The markers produced 58 bands with 57 identified as polymorphic. PIC values of the markers ranged between 0.01 and 0.44. Average PIC value was 0.28. Based on allele analysis with Darwin5 and STRUCTURE tools, 176 individual were clustered into three groups. The first cluster mostly consisted of accessions from Europe and USA and contained 69 samples. The second cluster mostly consisted of lines from Asia and neighboring countries and contained 89 samples. The third cluster did not represent any characteristic according to geographic region, thus it was called an intermixed cluster which contained 18 samples. The maximum genetic dissimilarity of spinach accessions was 0.551 and minimum was 0.019. Average genetic dissimilarity was 0.217. Moreover we sequenced S. oleracea L. cv. Universal nuclear genome via IIIumina MiSeq technology and genome assembly was performed to develop new spinach–specific SSR markers. As a result, 3853 SSRs were identified in the assembled genome and we successfully designed 3275 primer pairs for these identified SSR motifs. These newly developed SSR markers will be helpful to scientists who are interested in spinach genome diversity and breeding.en_US
dc.description.abstractIspanak (Spinacia oleracea L.) Amaranthaceae ailesine ait olan popüler ve besleyici bir sebzedir. Bu yeşil yapraklı bitkinin Persia (İran ve komşu ülkelerden) kökenlendiğine inanılır. Bu çalışmada Dünya Koleksiyonuna ait 176 ıspanak çeşidi, bilinen 15 SSR markır ile taranmıştır. Markırlar 57’si polimorfik olmak üzere 58 bant üretmiştir. Markırların PIC değerleri 0.01 ile 0.44 arasında değişmektedir. Ortalama PIC değeri ise 0.28’dir. Darwin5 ve STRUCTURE programlarının allel analizlerine göre 176 birey üç grupta toplanmıştır. Birinci grupta çoğunlukla Amerika ve Avrupa çeşitleri olmak üzere 69 örnek birey vardır. İkinci grup ise çoğunlukla Asya ve komşu ülkelerden olmak üzere 89 birey vardır. Üçüncü grup coğrafi alana göre hiç bir karakteristik göstermemiştr, bu nedenle 18 birey içeren bu grup intermixed grup olarak isimlendirilmiştir. Ispanak çeşitlerinin genetik farklılığı maksimum 0.051 ve minum 0.019’dur. Ortalama genetik farklılık ise 0.217’dir. Ayrica S. oleracea L. cv. Universal nuklear genomu IIIumina MiSeq teknolojisi kullanılarak dizilenip, ıspanak-spesifik SSR markır dizaynı için genom montajı yapıldı. Sonuç olarak, montajlanan genomda 3852 SSRs tespit edildi ve 3275 primer çifti, tespit edilen SSR motiflerine başarılı olarak dizayn edildi. Bu yeni geliştirilen SSR markırlar, ıspanak genom çeşitliliği ve ıslah konusu ile ilgilenen bilim adamlarına yardımcı olacaktır.en_US
dc.language.isoenen_US
dc.publisherIzmir Institute of Technologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenetic markersen_US
dc.subjectSpinach (Spinacia oleracea L.)en_US
dc.subjectPlant molecular geneticsen_US
dc.titleCharacterization of world spinach genetic collection by using molecular markersen_US
dc.title.alternativeDünya ıspanak genetik kolleksiyonlarının moleküler markörlerle karakterizasyonuen_US
dc.typeMaster Thesisen_US
dc.institutionauthorGöktay, Mehmet-
dc.departmentThesis (Master)--İzmir Institute of Technology, Molecular Biology and Geneticsen_US
dc.relation.publicationcategoryTezen_US
item.openairetypeMaster Thesis-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept01. Izmir Institute of Technology-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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