Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4670
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dc.contributor.authorWorthington, Erin N.-
dc.contributor.authorKavaklı, İ. Halil-
dc.contributor.authorBerrocal-Tito, Gloria M.-
dc.contributor.authorBondo, Bruce-
dc.contributor.authorSancar, Aziz-
dc.date.accessioned2016-05-27T10:32:15Z
dc.date.available2016-05-27T10:32:15Z
dc.date.issued2003-10
dc.identifier.citationWorthington, E. N., Kavaklı, İ. H., Berrocal-Tito, G. M., Bondo, B., and Sancar, A. (2003). Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from vibrio cholerae. Journal of Biological Chemistry, 278(40), 39143-39154. doi:10.1074/jbc.M305792200en_US
dc.identifier.issn0021-9258
dc.identifier.issn0021-9258-
dc.identifier.urihttp://doi.org/10.1074/jbc.M305792200
dc.identifier.urihttp://hdl.handle.net/11147/4670
dc.description.abstractThe sequence of Vibrio cholerae genome revealed three genes belonging to the photolyase/cryptochrome blue-light photoreceptor family. The proteins encoded by the three genes were purified and characterized. All three proteins contain folate and flavin cofactors and have absorption peaks in the range of 350-500 nm. Only one of the three, VcPhr, is a photolyase specific for cyclobutane pyrimidine dimers. The other two are cryptochromes and were designated VcCry1 and VcCry2, respectively. Mutation of phr abolishes photoreactivation of UV-induced killing, whereas mutations in cry1 and cry2 do not affect photorepair activity. VcCry1 exhibits some unique features. Of all cryptochromes characterized to date, it is the only one that contains stoichiometric amounts of both chromophores and retains its flavin cofactor in the two-electron reduced FADH2 form. In addition, VcCry1 exhibits RNA binding activity and copurifies with an RNA of 60-70 nucleotides in length.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPhotoreceptorsen_US
dc.subjectDimersen_US
dc.subjectMutagenesisen_US
dc.subjectProteinsen_US
dc.subjectNegibacteriaen_US
dc.subjectRNAen_US
dc.titlePurification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from vibrio choleraeen_US
dc.typeArticleen_US
dc.institutionauthorKavaklı, İ. Halil-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume278en_US
dc.identifier.issue40en_US
dc.identifier.startpage39143en_US
dc.identifier.endpage39154en_US
dc.identifier.wosWOS:000185575100128en_US
dc.identifier.scopus2-s2.0-0141531996en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1074/jbc.M305792200-
dc.identifier.pmid12878596en_US
dc.relation.doi10.1074/jbc.M305792200en_US
dc.coverage.doi10.1074/jbc.M305792200en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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