Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4729
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dc.contributor.authorYavuz, Elif-
dc.contributor.authorGüneş, Hatice-
dc.contributor.authorHarsa, Hayriye Şebnem-
dc.contributor.authorYenidünya, Ali Fazıl-
dc.date.accessioned2016-06-07T07:00:24Z-
dc.date.available2016-06-07T07:00:24Z-
dc.date.issued2004-
dc.identifier.citationYavuz, E., Güneş, H., Harsa, H. Ş., and Yenidünya, A. F. (2004). Identification of extracellular enzyme producing thermophilic bacilli from Balcova (Agamemnon) geothermal site by ITS rDNA RFLP. Journal of Applied Microbiology, 97(4), 810-817. doi:10.1111/j.1365-2672.2004.02374.xen_US
dc.identifier.issn1364-5072-
dc.identifier.issn1365-2672-
dc.identifier.issn1364-5072-
dc.identifier.urihttp://doi.org/10.1111/j.1365-2672.2004.02374.x-
dc.identifier.urihttp://hdl.handle.net/11147/4729-
dc.description.abstractAims: Molecular characterization of extracellular enzyme producing thermophilic bacilli from Balcova geothermal site. Methods and Results: Three types of geothermal samples were collected: mud, re-injection water, and samples from uncontrolled hydrothermal vents. Isolates grown at 55°C in culture media prepared in sterilized re-injection water, were screened for extracellular enzyme activity by using eight different substrates: casein, carboxymethyl-cellulose, pectin, polygalacturonic acid (PGA), soluble starch, Tween 20 and 80, and xylan. In total, 109 thermoaerophilic isolates were selected. All of the isolates could hydrolyse Tween 20 (100%) but not Tween 80. Soluble starch was hydrolysed by 96%, casein by 55%, xylan and carboxymethylcellulose by 9%, and pectin and PGA by 2% of the isolates. The isolates were grouped into 14 different homology groups by the restriction pattern analysis of 16S-internal transcribed spacer (ITS) rDNA RFLP. Each of the RFLP groups was also studied by 16S rRNA gene partial sequence analysis. Plasmid DNA profiles revealed that 15 of the isolated strains contained small plasmid DNA molecules ranging in size from 12 000 to 35 000 bp. Conclusions: Combined analysis of 16S-ITS rDNA RFLP and 16S rRNA gene partial sequence results indicated the presence of novel or existing species of Anoxybacillus (nine species) and Geobacillus (three species). Significance and Impact of the Study: In this study 16S-ITS rDNA RFLP was applied for the first time to differentiate thermophilic bacilli. It was also the first study on thermophilic bacilli of Balcova geothermal site.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofJournal of Applied Microbiologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject16S-ITS rDNA RFLPen_US
dc.subjectExtracellular enzyme screeningen_US
dc.subjectGeothermalen_US
dc.subjectSimilarity analysisen_US
dc.subjectThermophilic bacillien_US
dc.subjectAnoxybacillusen_US
dc.titleIdentification of extracellular enzyme producing thermophilic bacilli from Balcova (Agamemnon) geothermal site by ITS rDNA RFLPen_US
dc.typeArticleen_US
dc.authoridTR2082en_US
dc.authoridTR9626en_US
dc.authoridTR42080en_US
dc.institutionauthorYavuz, Elif-
dc.institutionauthorGüneş, Hatice-
dc.institutionauthorHarsa, Hayriye Şebnem-
dc.institutionauthorYenidünya, Ali Fazıl-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume97en_US
dc.identifier.issue4en_US
dc.identifier.startpage810en_US
dc.identifier.endpage817en_US
dc.identifier.wosWOS:000223817600017en_US
dc.identifier.scopus2-s2.0-5044228263en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1111/j.1365-2672.2004.02374.x-
dc.identifier.pmid15357731en_US
dc.relation.doi10.1111/j.1365-2672.2004.02374.xen_US
dc.coverage.doi10.1111/j.1365-2672.2004.02374.xen_US
local.message.claim2022-06-03T16:38:19.997+0300|||rp02904|||submit_approve|||dc_contributor_author|||None*
local.message.claim2022-06-16T10:28:54.985+0300|||rp03047|||submit_approve|||dc_contributor_author|||None*
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.openairetypeArticle-
crisitem.author.dept01. Izmir Institute of Technology-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept03.08. Department of Food Engineering-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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