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Title: Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds
Authors: Vennapusa, Rami Reddy
Tarı, Canan
Cabrera, Rosa
Fernandez-Lahore, Marcelo
Keywords: Biomass attachment
Bioprocess design
Direct sequestration
Fluidized beds
Hydrophobic interaction
Sorption performance
Publisher: Elsevier Ltd.
Source: Vennapusa, R. R., Tarı, C., Cabrera, R., and Fernandez-Lahore, M. (2009). Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds. Biochemical Engineering Journal, 43(1), 16-26. doi:10.1016/j.bej.2008.08.004
Abstract: Cell-to-support interaction and cell-to-cell aggregation phenomena have been studied in a model system composed of intact yeast cells and Phenyl-Streamline adsorbents. Biomass components and beaded adsorbents were characterized by contact angle determinations with three diagnostic liquids and zeta potential measurements. Subsequently, free energy of interaction vs. distance profiles between interacting surfaces was calculated in the aqueous media provided by operating mobile phases. The effect of pH and ammonium sulphate concentration within the normal operating ranges was evaluated. Calculation indicated that moderate interaction between cell particles and adsorbent beads can develop in the presence of salt. Cell-to-cell aggregation was suspected to occur at high salt concentration and neutral pH. Predictions based on the application of the XDLVO approach were confirmed by independent experimental methods like biomass deposition experiments and laser diffraction spectroscopy. Understanding biomass attachment onto hydrophobic supports can help in alleviating process limitations normally encountered during expanded bed adsorption of bioproducts.
ISSN: 1369-703X
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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