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Title: Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C 4) and Cleome spinosa (C 3) under drought stress
Authors: Uzilday, Barış
Türkan, İsmail
Sekmen, Aşkım Hediye
Özgür, Rengin
Karakaya, Hüseyin Çağlar
Keywords: Antioxidant enzymes
Cleome gynandra
Cleome spinosa
Drought analysis
Issue Date: Jan-2012
Publisher: Elsevier Ltd.
Source: Uzilday, B., Türkan, İ., Sekmen, A. H., Özgür, R., and Karakaya, H. Ç. (2012). Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C 4) and Cleome spinosa (C 3) under drought stress. Plant Science, 182(1), 59-70. doi:10.1016/j.plantsci.2011.03.015
Abstract: Differences between antioxidant responses to drought in C 3 and C 4 plants are rather scanty. Even, we are not aware of any research on comparative ROS formation and antioxidant enzymes in C 3 and C 4 species differing in carboxylation pathway of same genus which would be useful to prevent other differences in plant metabolism. With this aim, relative shoot growth rate, relative water content and osmotic potential, hydrogen peroxide (H 2O 2) content and NADPH oxidase (NOX) activity, antioxidant defence system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR) enzymes and their isoenzymes), CAT1 mRNA level, and lipid peroxidation in seedlings of Cleome spinosa (C 3) and Cleome gynandra (C 4) species of Cleome genus exposed to drought stress for 5 and 10 day (d) were comparatively investigated. Constitutive levels of antioxidant enzymes (except SOD) were consistently higher in C. spinosa than in C. gynandra under control conditions. CAT1 gene expression in C. spinosa was correlated with CAT activity but CAT1 gene expression in C. gynandra at 10 d did not show this correlation. Drought stress caused an increase in POX, CAT, APX and GR in both species. However, SOD activity was slightly decreased in C. gynandra while it was remained unchanged or increased on 5 and 10 d of stress in C. spinosa, respectively. Parallel to results of malon dialdehyde (MDA), H 2O 2 content was also remarkably increased in C. spinosa as compared to C. gynandra under drought stress. These results suggest that in C. spinosa, antioxidant defence system was insufficient to suppress the increasing ROS production under stress condition. On the other hand, in C. gynandra, although its induction was lower as compared to C. spinosa, antioxidant system was able to cope with ROS formation under drought stress. © 2011 Elsevier Ireland Ltd.
ISSN: 0168-9452
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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