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Title: Identification of respiratory chain gene mutations that shorten replicative life span in yeast
Authors: Hacıoğlu, Elise
Demir, Ayşe Banu
Koç, Ahmet
Keywords: Aging
Chronological aging
Electron transport chain
Reactive oxygen species
Replicative life span
Issue Date: Feb-2012
Publisher: Elsevier Ltd.
Source: Hacıoğlu, E., Demir, A. B., and Koç, A. (2012). Identification of respiratory chain gene mutations that shorten replicative life span in yeast. Experimental Gerontology, 47(2), 149-153. doi:10.1016/j.exger.2011.11.009
Abstract: Aging is the progressive accumulation of alterations in cells that elevates the risk of death. The mitochondrial theory of aging postulates that free radicals produced by the mitochondrial respiratory system contribute to the aging process. However, the roles of individual electron transfer chain (ETC) components in cellular aging have not been elucidated. In this study, we analyzed the replicative life span of 73 yeast deletion mutants lacking the genes of the mitochondrial electron transfer chain system, and found that nine of these mutants (δ nde1, δ tcm62, δ rip1, δ cyt1, δ qrc8, δ pet117, δ cox11, δ atp11, δ fmc1) had significantly shorter life spans. These mutants had lower rates of respiration and were slightly sensitive to exogenous administration of hydrogen peroxide. However, only two of them, δ nde1 and δ fmc1, produced higher amounts of intrinsic superoxide radicals in the presence of glucose compared to that of wild type cells. Interestingly, there were no significant alterations in the mitochondrial membrane potentials of these mutants. We speculate that the shorter life spans of ETC mutants result from multiple mechanisms including the low respiration rate and low energy production rather than just a ROS-dependent path. © 2011 Elsevier Inc.
ISSN: 0531-5565
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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