Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4899
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dc.contributor.authorKow, Siew Ching-
dc.contributor.authorMccorroll, Joshua A.-
dc.contributor.authorValade, David-
dc.contributor.authorBoyer, Cyrille-
dc.contributor.authorDwarte, Tanya-
dc.contributor.authorDavis, Thomas P.-
dc.contributor.authorKavallaris, Maria-
dc.contributor.authorBulmuş, Volga-
dc.date.accessioned2017-02-24T07:56:53Z-
dc.date.available2017-02-24T07:56:53Z-
dc.date.issued2011-12-12-
dc.identifier.citationKow, S. C., McCarroll, J., Valade, D., Boyer, C., Dwarte, T., Davis, T. P., Kavallaris, M., and Bulmus, V. (2011). Dicer-labile PEG conjugates for siRNA delivery. Biomacromolecules, 12(12), 4301-4310. doi:10.1021/bm201199cen_US
dc.identifier.issn1526-4602-
dc.identifier.issn1525-7797-
dc.identifier.urihttp://doi.org/10.1021/bm201199c-
dc.identifier.urihttp://hdl.handle.net/11147/4899-
dc.description.abstractPoly(ethylene glycol) (PEG) conjugates of Dicer-substrate small interfering RNA (DsiRNA) have been prepared to investigate a new siRNA release strategy. 3'-sense or 5'-antisense thiol-modified, blunt-ended DsiRNAs, inhibiting enhanced green fluorescent protein (eGFP) expression, were covalently conjugated to PEG with varying molecular weights (2, 10, and 20 kg/mol) through a stable thioether bond using a Michael addition reaction. The DsiRNA conjugates with 2 kg/mol PEG (both 3'-sense or 5'-antisense strand conjugated) and the 10 kg/mol PEG conjugated to the 3'-sense strand of DsiRNA were efficiently cleaved by recombinant human Dicer to 21-mer siRNA, as determined by gel electrophoresis. Importantly, 2 and 10 kg/mol PEG conjugated to the 3'-sense strand of DsiRNA showed potent gene silencing activity in human neuroblastoma (SH-EP) cells, stably expressing eGFP, at both the mRNA and protein levels. Moreover, the 10 kg/mol PEG conjugates of the 3'-sense strand of DsiRNA were less immunogenic when compared with the unmodified DsiRNA, determined via an immune stimulation assay on human peripheral blood mononuclear cells.en_US
dc.description.sponsorshipAustralian Research Council (ARC) (DP 0770818)en_US
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofBiomacromoleculesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntisenseen_US
dc.subjectEnhanced green fluorescent proteinen_US
dc.subjectGel electrophoresisen_US
dc.subjectGene silencingen_US
dc.subjectHuman neuroblastomaen_US
dc.subjectAddition reactionsen_US
dc.subjectsiRNA deliveryen_US
dc.titleDicer-labile PEG conjugates for siRNA deliveryen_US
dc.typeArticleen_US
dc.authoridTR181383en_US
dc.institutionauthorBulmuş, Volga-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume12en_US
dc.identifier.issue12en_US
dc.identifier.startpage4301en_US
dc.identifier.endpage4310en_US
dc.identifier.wosWOS:000297782100016en_US
dc.identifier.scopus2-s2.0-84856295099en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1021/bm201199c-
dc.identifier.pmid22053777en_US
dc.relation.doi10.1021/bm201199cen_US
dc.coverage.doi10.1021/bm201199cen_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairetypeArticle-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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